Journal Article

Protective effects of Pudilan Tablets against osteoarthritis in mice induced by monosodium iodoacetate

Feng, Shibin, Han, Rongchun, Zhou, Chenyu, Fang, Zhizheng, Lei, Shujun, Li, Xiangyu, Tong, Xiaohui

2023

Overview

Osteoarthritis (OA) is a complicated disorder that is the most prevalent chronic degenerative joint disease nowadays. Pudilan Tablets (PDL) is a prominent traditional Chinese medicine formula used in clinical settings to treat chronic inflammatory illnesses. However, there is currently minimal fundamental research on PDL in the therapy of joint diseases. As a result, this study looked at the anti-inflammatory and anti-OA properties of PDL in vitro and in vivo, as well as the mechanism of PDL in the treatment of OA. We investigated the anti-OA properties of PDL in OA mice that were generated by monosodium iodoacetate (MIA). All animals were administered PDL (2 g/kg or 4 g/kg) or the positive control drug, indomethacin (150 mg/kg), once daily for a total of 28 days starting on the day of MIA injection. The CCK-8 assay was used to test the vitality of PDL-treated RAW264.7 cells in vitro. RAW264.7 cells that had been activated with lipopolysaccharide (LPS) were used to assess the anti-inflammatory properties of PDL. In the MIA-induced OA model mice, PDL reduced pain, decreased OA-induced cartilage damages and degradation, decreased production of pro-inflammatory cytokines in serum, and suppressed IL-1β , IL-6 , and TNF-α mRNA expression levels in tibiofemoral joint. In RAW264.7 cells, PDL treatment prevented LPS-induced activation of the ERK/Akt signaling pathway and significantly decreased the levels of inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. In conclusion, these results suggest that PDL is involved in combating the development and progression of OA, exerts a powerful anti-inflammatory effect on the knee joint, and may be a promising candidate for the treatment of OA.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/154/436 / 631/45/127/1213 / 631/45/127/1220 / AKT protein / Animal models / Animals / Anti-inflammatory agents / Anti-Inflammatory Agents - pharmacology / Arthritis / Cartilage diseases / Cartilage, Articular - metabolism / Cholecystokinin / Cytokines / Disease Models, Animal / Drugs, Chinese Herbal - pharmacology / Gene expression / Herbal medicine / Humanities and Social Sciences / IL-1β / Indomethacin / Inflammation / Interleukin 6 / Interleukin-6 - metabolism / Iodoacetic Acid - toxicity / Joint diseases / Lipopolysaccharides / Mice / multidisciplinary / Osteoarthritis / Osteoarthritis - chemically induced / Osteoarthritis - drug therapy / Osteoarthritis - metabolism / RAW 264.7 Cells / Science / Science (multidisciplinary) / Signal transduction / Tablets / Traditional Chinese medicine / Tumor Necrosis Factor-alpha - metabolism / Tumor necrosis factor-α