Journal Article

Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo

Wenzel, Michael, Bando, Yuki, Yuste, Rafael

2021

Overview

To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals. The authors present a geneticallyencoded voltage indicator to specifically measure subthreshold membrane potentials. They combine two-photon imaging of voltage and calcium to map epileptic seizures progression through cortical circuits.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 14 / 14/35 / 14/69 / 631/1647/1888/2249 / 631/1647/245/2226 / 631/378/3920 / 9/10 / 9/30 / 9/74 / Action Potentials - physiology / Animals / Calcium / Calcium channels (voltage-gated) / Calcium imaging / Circuits / Convulsions & seizures / Epilepsy / Female / Green Fluorescent Proteins / Humanities and Social Sciences / Imaging / In vivo methods and tests / Male / Mapping / Membrane Potentials - physiology / Membranes / Mice / Mice, Inbred ICR / multidisciplinary / Neurons - physiology / Optical Imaging - methods / Photons / Populations / Science / Science (multidisciplinary) / Seizures / Voltage indicators