Journal Article

Elevated protein synthesis in microglia causes autism-like synaptic and behavioral aberrations

Sun, Ye, Xu, Zhi-Xiang, Lee, Chan Hee, Tan, Ji-Wei, Lee, Sang-Hoon, Xu, Ethan Y., Liao, Guey-Ying, Xu, Haifei, Clipperton-Allen, Amy E., Kwon, Soonwook, Xu, Baoji, Riso, Anna E., Kim, Gyu Hyun, Lee, Kea Joo, Do, Na-Young, Page, Damon T.

2020

Overview

Mutations that inactivate negative translation regulators cause autism spectrum disorders (ASD), which predominantly affect males and exhibit social interaction and communication deficits and repetitive behaviors. However, the cells that cause ASD through elevated protein synthesis resulting from these mutations remain unknown. Here we employ conditional overexpression of translation initiation factor eIF4E to increase protein synthesis in specific brain cells. We show that exaggerated translation in microglia, but not neurons or astrocytes, leads to autism-like behaviors in male mice. Although microglial eIF4E overexpression elevates translation in both sexes, it only increases microglial density and size in males, accompanied by microglial shift from homeostatic to a functional state with enhanced phagocytic capacity but reduced motility and synapse engulfment. Consequently, cortical neurons in the mice have higher synapse density, neuroligins, and excitation-to-inhibition ratio compared to control mice. We propose that functional perturbation of male microglia is an important cause for sex-biased ASD. The main cell types involved in autism spectrum disorders through elevated protein synthesis are not well identified. Here, the authors show that overexpression of translation initiation factor eIF4E in microglia results in autism-like behaviour in male, but not female, mice.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/51 / 14/19 / 14/28 / 14/63 / 38/91 / 631/378/1689/1373 / 631/378/340 / 631/378/87 / 64/60 / Animals / Astrocytes / Autism / Autistic Disorder - metabolism / Behavior, Animal / Calcium-Binding Proteins - metabolism / Cell interactions / Cell Movement / Density / Disorders / Female / Gene Expression Profiling / Genotype / Homeostasis / Humanities and Social Sciences / Initiation factor eIF-4E / Male / Males / Mice, Knockout / Microfilament Proteins - metabolism / Microglia / Microglia - metabolism / multidisciplinary / Mutation / Neurons / Neurons - metabolism / Perturbation / Phagocytes / Phagocytosis / Prefrontal Cortex - metabolism / Prefrontal Cortex - ultrastructure / Protein Biosynthesis / Protein synthesis / Proteins / PTEN Phosphohydrolase - metabolism / Regulators / Science / Science (multidisciplinary) / Social Behavior / Social factors / Synapses / Synapses - metabolism / Synaptic density