Asset Details
Do you wish to reserve the book?
PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2
Do you wish to request the book?
PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2
2021
Overview
Cardiomyocytes undergo significant structural and functional changes after birth, and these fundamental processes are essential for the heart to pump blood to the growing body. However, due to the challenges of isolating single postnatal/adult myocytes, how individual newborn cardiomyocytes acquire multiple aspects of the mature phenotype remains poorly understood. Here we implement large-particle sorting and analyze single myocytes from neonatal to adult hearts. Early myocytes exhibit wide-ranging transcriptomic and size heterogeneity that is maintained until adulthood with a continuous transcriptomic shift. Gene regulatory network analysis followed by mosaic gene deletion reveals that peroxisome proliferator-activated receptor coactivator-1 signaling, which is active in vivo but inactive in pluripotent stem cell-derived cardiomyocytes, mediates the shift. This signaling simultaneously regulates key aspects of cardiomyocyte maturation through previously unrecognized proteins, including YAP1 and SF3B2. Our study provides a single-cell roadmap of heterogeneous transitions coupled to cellular features and identifies a multifaceted regulator controlling cardiomyocyte maturation.
Cardiomyocyte maturation and the acquisition of phenotypes is poorly understood at the single cell level. Here, the authors analyse the transcriptome of single cells from neonatal to adult heart and reveal that peroxisome proliferator-activated receptor coactivator-1 mediates the phenotypic shift.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/100
/ 38
/ 38/90
/ 38/91
/ 45
/ 45/91
/ 64
/ 64/60
/ Adaptor Proteins, Signal Transducing - metabolism
/ Animals
/ Cell Cycle Proteins - genetics
/ Cell Cycle Proteins - metabolism
/ Heart
/ Humanities and Social Sciences
/ Humans
/ Induced Pluripotent Stem Cells - metabolism
/ Mice
/ Myocytes
/ Myocytes, Cardiac - metabolism
/ Neonates
/ Peroxisome proliferator-activated receptors
/ Peroxisome Proliferator-Activated Receptors - genetics
/ Peroxisome Proliferator-Activated Receptors - metabolism
/ Pluripotent Stem Cells - metabolism
/ RNA Splicing Factors - metabolism
/ Science
/ Structure-function relationships
/ Transcription Factors - genetics
