Asset Details
Do you wish to reserve the book?
Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton
Do you wish to request the book?
Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton
2023
Overview
Senescence drives organismal aging, yet the deep characterization of senescent cells in vivo remains incomplete. Here, we apply mass cytometry by time-of-flight using carefully validated antibodies to analyze senescent cells at single-cell resolution. We use multiple criteria to identify senescent mesenchymal cells that are growth-arrested and resistant to apoptosis. These p16 + Ki67-BCL-2+ cells are highly enriched for senescence-associated secretory phenotype and DNA damage markers, are strongly associated with age, and their percentages are increased in late osteoblasts/osteocytes and CD24
high
osteolineage cells. Moreover, both late osteoblasts/osteocytes and CD24
high
osteolineage cells are robustly cleared by genetic and pharmacologic senolytic therapies in aged mice. Following isolation, CD24+ skeletal cells exhibit growth arrest, senescence-associated β-galactosidase positivity, and impaired osteogenesis in vitro. These studies thus provide an approach using multiplexed protein profiling to define senescent mesenchymal cells in vivo and identify specific skeletal cell populations cleared by senolytics.
Technical challenges have previously hindered the detailed study of in vivo senescent cells. Here, the authors deeply characterize senescent skeletal cells across murine aging, establishing CD24 as a marker of osteolineage cells cleared by senolytics.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/91
/ 64/60
/ 82/58
/ Aging
/ Animals
/ Cellular Senescence - genetics
/ Humanities and Social Sciences
/ Mice
/ Science
/ Skeleton
We currently cannot retrieve any items related to this title. Kindly check back at a later time.