Journal Article

Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor

Kuang, Hua, Liu, Liqiang, Xing, Changrui, Hao, Changlong, Wang, Libing, Xu, Chuanlai

2013

Overview

In this study, we have first developed a rapid and sensitive strip immunosensor based on two heterogeneously-sized gold nanoparticles (Au NPs) probes for the detection of trace lead ions in drinking water. The sensitivity was 4-fold higher than that of the conventional LFA under the optimized conditions. The visual limit of detection (LOD) of the amplified method for qualitative detection lead ions was 2 ng/mL and the LOD for semi-quantitative detection could go down to 0.19 ng/mL using a scanning reader. The method suffered from no interference from other metal ions and could be used to detect trace lead ions in drinking water without sample enrichment. The recovery of the test samples ranged from 96% to 103%. As the detection method could be accomplished within 15 min, this method could be used as a potential tool for preliminary monitoring of lead contamination in drinking water.

• Publisher: MDPI AG,Molecular Diversity Preservation International (MDPI)
• Subject: amplified competitive lateral flow assay / Animals / Antibodies, Monoclonal - metabolism / Drinking water / Drinking Water - chemistry / Enrichment / Food contamination & poisoning / Food science / Gold / gold nanoparticles / Heavy metals / Immunoassay - methods / immunosensor / Immunosensors / Ions / Laboratories / Lead (metal) / Lead - analysis / Lead content / lead ions / Methods / Mice / Mice, Inbred BALB C / Monoclonal antibodies / Nanoparticles / Proteins / Readers / Reagent Strips - chemistry / Reproducibility of Results / Scientific imaging / Sensors / Strip