Journal Article

High levels of multiresistance in quinolone resistant urinary tract isolates of Escherichia coli from Norway; a non clonal phenomen?

Jenkins, Andrew, Grude, Nils, Strand, Linda, Henriksen, Ingrid Høgli, Allum, Anne Gry, Kristiansen, Bjørn Erik

2014

Overview

Background The problem of emerging ciprofloxacin resistance is compounded by its frequent association with multiresistance, the reason for which is not fully understood. In this study we compare multiresistance, clonal similarities and phylogenetic group in urinary tract isolates of Escherichia coli sensitive and resistant to the quinolone antimicrobials nalidixic acid and ciprofloxacin. Results Quinolone resistant isolates were more resistant to non-quinolone antibiotics than sensitive isolates, with resistance to ampicillin, mecillinam, sulphonamide, trimethoprim, tetracycline, kanamycin and chloramphenicol significantly increased. Fifty-one percent of quinolone-resistant isolates were multiresistant. Although multiresistance was most prevalent (63%) in isolates showing high-level ciprofloxacin resistance, it was still highly prevalent (41%) in nalidixic acid resistant isolates with low-level ciprofloxacin resistance. Multiresistance was more frequent among singleton isolates (61%) than clonal isolates (40%) of quinolone resistant Escherichia coli. Ciprofloxacin resistance was associated with certain specific clones, among them the globally distributed clonal Group A. However, there was no significant difference in the overall degree of clonality between quinolone sensitive and resistant isolates. Ciprofloxacin resistance was positively associated with phylogroup D and negatively associated with phylogroup B2. This correlation was not associated with clonal isolates. Conclusion This study supports earlier findings of association between ciprofloxacin resistance and resistance to other antibiotics. The prevalence of multiresistance in quinolone-resistant isolates that have not yet developed high-level ciprofloxacin resistance suggest that multiresistance arises early in the development of quinolone resistance. This is consistent with exposure to quinolones causing quinolone resistance by mutations and mobilization of multiresistance elements by induction of the SOS response. The spread of clones seems to be less important than previously reported in regard to emergence of quinolone resistance and multiresistance as both are associated primarily with singleton isolates.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V
• Subject: Acids / Ampicillin / Analysis / Anti-Bacterial Agents - pharmacology / Antibiotics / Aquifers / Biomedical and Life Sciences / Biomedicine / Ciprofloxacin / Ciprofloxacin - pharmacology / Cloning / Drinking water / Drug resistance in microorganisms / Drug Resistance, Multiple, Bacterial - drug effects / Drug Resistance, Multiple, Bacterial - genetics / E coli / Escherichia coli / Escherichia coli - classification / Escherichia coli - genetics / Escherichia coli - isolation & purification / Escherichia coli Infections - microbiology / Genetic aspects / Health aspects / Humans / Life Sciences / Medicine/Public Health / Microbial Sensitivity Tests / Mutation / Nalidixic Acid - pharmacology / Norway / Phylogeny / Physiological aspects / Quinolones - pharmacology / Research Article / Species Specificity / Sulfonamides / Urinary Tract - microbiology / Urogenital system