Journal Article

The skimmed milk proteome of dairy cows is affected by the stage of lactation and by supplementation with polyunsaturated fatty acids

Universität Bonn = University of Bonn, Institut de Mathématiques de Toulouse UMR5219 (IMT) ; Université Toulouse Capitole (UT Capitole) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse - Jean Jaurès (UT2J) ; Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Viala, Didier, Ceciliani, Fabrizio, Università degli Studi di Milano = University of Milan (UNIMI), Delosière, Mylène, Sauerwein, Helga, Vogel, Laura, Hammon, Harald, M, Bonnet, Muriel, Tröscher, Arnulf, Déjean, Sébastien, BASF SE, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH) ; VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, scienc

2024

Overview

Abstract The impact of nutritional modification to increase functional polyunsaturated fatty acids (PUFA), such as n-3 and n-6 fatty acids (FA) or conjugated linoleic acid (CLA), on milk proteome profile during early lactation remains largely unknown. We used an untargeted proteomics approach to investigate the impact of lactation day and PUFA supplementation on the proteome signature in skimmed milk over the course of early lactation. Sixteen Holstein dairy cows received abomasal infusion of saturated FA (CTRL) or a mixture of essential FA and CLA (EFA + CLA group) from − 63 to + 63 days relative to parturition. Using quantitative proteomics, 479 unique proteins were identified in skimmed milk at days 1, 28, and 63 postpartum. The top discriminating proteins between transition milk (day 1) and mature milk (days 28 and 63), including members of complements (i.e. C2 and C5), growth factor (TGFB2), lipoproteins (i.e. APOE and APOD), and chaperones (i.e. ST13 and CLU), are associated with calves’ immune system and gut development. The EFA + CLA supplementation moderately affected a few proteins associated with regulating mammary glands’ lipogenesis through the (re)assembly of lipoprotein particles, possibly under the PPAR signaling pathway. Collectively, skimmed milk proteome is dynamically regulated initially by cow’s metabolic and physiological changes and to a lesser extent by nutritional PUFA modifications.

• Publisher: Nature Publishing Group,CCSD,Nature Publishing Group UK,Nature Portfolio
• Subject: 631/114 / 631/1647 / 631/337 / 631/443 / 631/601 / Animal lactation / Animals / Apolipoprotein E / Cattle / Cow's milk / Dairy cattle / Dietary Supplements / Fatty acids / Fatty Acids, Unsaturated / Fatty Acids, Unsaturated - metabolism / Female / Food and Nutrition / Humanities and Social Sciences / Immune system / Lactation / Life Sciences / Linoleic acid / Linoleic Acids, Conjugated / Linoleic Acids, Conjugated - metabolism / Linoleic Acids, Conjugated - pharmacology / Lipogenesis / Lipoproteins / Mammary gland / Milk / Milk - chemistry / Milk - metabolism / Milk Proteins / Milk Proteins - metabolism / multidisciplinary / Parturition / Polyunsaturated fatty acids / Proteins / Proteome / Proteome - metabolism / Proteomes / Proteomics / Proteomics - methods / Science / Science (multidisciplinary) / Signal transduction
• ISBN: 0013414863000