Journal Article

Astragali radix vesicle-like nanoparticles improve energy metabolism disorders by repairing the intestinal mucosal barrier and regulating amino acid metabolism in sleep-deprived mice

Liu, Jianxun, Song, Guochao, Hu, Longxiao, Zhang, Qiuyan, Ren, Junguo, Shi, Yali, Zhang, Na, Gao, Wenjing, Yuan, Yue, Gao, Yunxiao, Jiang, Yunyao

2024

Overview

Background Sleep disorder is widespread and involves a variety of intricate factors in its development. Sleep deprivation is a manifestation of sleep disorder, can lead to energy metabolism disturbances, weakened immune system, and compromised body functions. In extreme situations, sleep deprivation can cause organ failure, presenting significant risks to human health. Purpose This study aimed to investigate the efficacy and mechanisms of Astragalus Radix vesicles-like nanoparticles (AR-VLNs) in counteracting the deleterious effects of sleep deprivation. Methods The ICR mice were divided into control, model, AR-VLNs high dose (equivalent to 20 g/kg crude drug), AR-VLNs low dose (equivalent to 10 g/kg crude drug), AR high dose (equivalent to 20 g/kg crude drug), and AR low dose (equivalent to 10 g/kg crude drug). The REM (rapid eye movement) sleep-deprivation model was established, and evaluations were conducted for motor function, antioxidant capacity, and energy metabolism indices. Moreover, CACO-2 cells damage was induced with lipopolysaccharide to evaluate the repairing ability of AR-VLNs on the intestinal cell mucosa by measuring permeability. Furthermore, metabolomics was employed to elucidate the mechanisms of AR-VLNs action. Results AR-VLNs were demonstrated to enhance the motor efficiency and antioxidant capacity in REM sleep-deprived mice, while also minimized pathological damage and restored the integrity of the intestinal mucosal barrier. In vitro experiments indicated the anti-inflammatory effect of AR-VLNs against LPS-induced cell damage. Additionally, metabolomic analysis linked these effects with regulation of the amino acid metabolic pathways. Further confirmation from molecular biology experiments revealed that the protective effects of AR-VLNs against the deleterious effects of REM sleep deprivation were associated with the restoration of the intestinal mucosal barrier and the enhancement of amino acid metabolism. Conclusion AR-VLNs administration effectively improved energy metabolism disorders in REM sleep deprived mice, by facilitating the repair of the intestinal mucosal barrier and regulating the amino acid metabolism. Graphical Abstract

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Amino acid metabolism / Amino Acids - metabolism / Analysis / Animals / Astragali Radix / Astragalus propinquus - chemistry / Bioenergetics / Biotechnology / Caco-2 Cells / Care and treatment / Chemistry / Chemistry and Materials Science / Diagnosis / Disease susceptibility / Drugs, Chinese Herbal - pharmacology / Energy metabolism / Energy Metabolism - drug effects / Health aspects / Humans / Intestinal barrier function / Intestinal Mucosa - drug effects / Intestinal Mucosa - metabolism / Male / Metabolomics / Mice / Mice, Inbred ICR / Molecular Medicine / Nanoparticles / Nanoparticles - chemistry / Nanotechnology / Sleep deprivation / Sleep Deprivation - drug therapy / Sleep Deprivation - metabolism