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ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
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ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
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Journal Article
ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
2020
Overview
The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.
Accurate serology testing is urgently needed to help diagnose SARS-CoV-2 infection. Here Valkenburg and colleagues use a luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19 and find ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Adult
/ Aged
/ Antibodies, Viral - immunology
/ Antigens
/ Antigens, Viral - immunology
/ Betacoronavirus - immunology
/ Biomedical and Life Sciences
/ Clinical Laboratory Techniques - methods
/ Coronavirus Infections - blood
/ Coronavirus Infections - diagnosis
/ Coronavirus Infections - immunology
/ Coronavirus Infections - virology
/ COVID-19
/ Female
/ Humans
/ Identification and classification
/ Male
/ Pneumonia, Viral - diagnosis
/ Pneumonia, Viral - immunology
/ Serology
