Asset Details

MbrlCatalogueTitleDetail

Do you wish to reserve the book?

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

by Broadbent, David , Stehbens, Samantha J , Tata, Purushothama Rao , Sapoznik, Etai , Pohlkamp, Theresa , Dean, Kevin M , Huh, Jaewon , Schmidt, Jens C , Kobayashi, Yoshihiko , Carisey, Alexandre F , Millett-Sikking, Alfred , Marín, Arnaldo , Azarova, Evgenia V , Lee, Kyung-Min , Arteaga, Carlos L , Chang, Bo-Jui , York, Andrew G , Ju, Robert J , Shepherd, Douglas P , Welf, Erik S , Kruithoff, Rory , Yang, Bin , Fiolka, Reto P , Doubrovinski, Konstantin , Hanker, Ariella B

in Animals / Cell Biology / Cells, Cultured / fluorescence / Humans / light-sheet / Mice / Microfluidic Analytical Techniques - instrumentation / microfluidics / microscopy / Microscopy, Confocal - instrumentation / Microscopy, Confocal - methods / optogenetics / particle tracking / Plasmids / Rats / Single-Cell Analysis - methods

2020

Yes Please

Hey, we have placed the reservation for you!

By the way, why not check out events that you can attend while you pick your title.

Oops! Something went wrong.

Looks like we were not able to place the reservation. Kindly try again later.

Are you sure you want to remove the book from the shelf?

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

2020

Confirm

Do you wish to request the book?

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

2020

Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy

How would you like to get it?

Submit

We have requested the book for you!

Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.

Oops! Something went wrong.

Looks like we were not able to place your request. Kindly try again later.

Journal Article

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

Broadbent, David,

Stehbens, Samantha J,

Tata, Purushothama Rao,

Sapoznik, Etai,

Pohlkamp, Theresa,

Dean, Kevin M,

Huh, Jaewon,

Schmidt, Jens C,

Kobayashi, Yoshihiko,

Carisey, Alexandre F,

Millett-Sikking, Alfred,

Marín, Arnaldo,

Azarova, Evgenia V,

Lee, Kyung-Min,

Arteaga, Carlos L,

Chang, Bo-Jui,

York, Andrew G,

Ju, Robert J,

Shepherd, Douglas P,

Welf, Erik S,

Kruithoff, Rory,

Yang, Bin,

Fiolka, Reto P,

Doubrovinski, Konstantin,

Hanker, Ariella B

2020

Overview

We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.

Share this book

Add to My Shelf

Publisher

eLife Science Publications, Ltd,eLife Sciences Publications, Ltd,eLife Sciences Publications Ltd

Subject

/ Humans

/ Mice