Journal Article

Dendritic cell chemotaxis in 3D under defined chemokine gradients reveals differential response to ligands CCL21 and CCL19

Haessler, Ulrike, Wu, Mingming, Swartz, Melody A, Pisano, Marco

2011

Overview

Dendritic cell (DC) homing to the lymphatics and positioning within the lymph node is important for adaptive immunity, and is regulated by gradients of CCL19 and CCL21, ligands for CCR7. Despite the importance of DC chemotaxis, it is not well understood how DCs interpret gradients of these chemokines in a complex 3D microenvironment. Here, we use a microfluidic device that allows rapid establishment of stable gradients in 3D matrices to show that DC chemotaxis in 3D can respond to CCR7 ligand gradients as small as 0.4%, which helps explain how DCs sense lymphatic vessels in an environment where broadcast distance for chemokine diffusion is hindered by convective flows into the vessel. Interestingly, DCs displayed similar sensitivities to both chemokines at small gradients ([less-than or equal to] 60 nM/mm), but migrated more efficiently towards higher gradients of CCL21, which unlike CCL19 binds strongly to matrix proteoglycans and signals without the need for internalization. Furthermore, cells preferentially migrated towards CCL21 when exposed to equal and opposite gradients of CCL21 and CCL19 simultaneously, even when matrix-binding of CCL21 was prevented. Although these ligands have similar binding affinity to CCR7, our results demonstrate that, in a 3D environment, CCL21 is a more potent directional cue for DC migration than CCL19. These findings provide new quantitative insight into DC chemotaxis in a physiological 3D environment and suggest how CCL19 and CCL21 may signal differently to fine-tune DC homing and positioning within the lymphatic system. These results also have broad relevance to other systems of cell chemotaxis, which remain poorly understood in the 3D context.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: adaptive immunity / Animals / Average speed / binding capacity / Binding sites / Biological Sciences / CC chemokine receptors / CCL19 protein / CCL21 protein / CCR7 protein / CCR7 receptor / Cell adhesion & migration / Cell migration / Cell Movement - drug effects / Cells / chemokine CCL19 / Chemokine CCL19 - metabolism / chemokine CCL21 / Chemokine CCL21 - metabolism / Chemokines / Chemotaxis / Chemotaxis - physiology / Collagens / Dendritic cells / Dendritic Cells - metabolism / Dendritic Cells - physiology / Diffusion / Female / Leukocyte migration / Leukocytes / Ligands / Lymph nodes / Lymph Nodes - metabolism / Lymphatic system / Mice / Mice, Inbred C57BL / Microenvironments / Microfluidic Analytical Techniques / Microfluidic devices / Microfluidics / Molecules / Proteoglycans / Receptors / Receptors, CCR7 - metabolism