Journal Article

Evolution of antibody immunity to SARS-CoV-2

Hatziioannou, Theodora, Schaefer-Babajew, Dennis, Muecksch, Frauke, Oliveira, Thiago Y., Jankovic, Mila, Finkin, Shlomo, Ramos, Victor, Gordon, Kristie, Caskey, Marina, Shimeliovich, Irina, Yee, Jim, Turroja, Martina, Jha, Divya, Cipolla, Melissa, Mendoza, Pilar, Bjorkman, Pamela J., Gaebler, Christian, Hurley, Arlene, Martinez-Delgado, Gustavo, Schmidt, Fabian, Tankelevich, Michael, Bram, Yaron, Zhao, Zhen, Nussenzweig, Michel C., Tokuyama, Minami, Breton, Gaëlle, Dizon, Juan, Unson-O’Brien, Cecille, Robbiani, Davide F., Gazumyan, Anna, Millard, Katrina G., Schwartz, Robert E., Bieniasz, Paul D., Mehandru, Saurabh, Viant, Charlotte, Weisblum, Yiska, Wang, Zijun, Lorenzi, Julio C. C., Cho, Alice, Patel, Roshni, Barnes, Christopher O., Hägglöf, Thomas

2021

Overview

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected 78 million individuals and is responsible for over 1.7 million deaths to date. Infection is associated with the development of variable levels of antibodies with neutralizing activity, which can protect against infection in animal models 1 , 2 . Antibody levels decrease with time, but, to our knowledge, the nature and quality of the memory B cells that would be required to produce antibodies upon reinfection has not been examined. Here we report on the humoral memory response in a cohort of 87 individuals assessed at 1.3 and 6.2 months after infection with SARS-CoV-2. We find that titres of IgM and IgG antibodies against the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 decrease significantly over this time period, with IgA being less affected. Concurrently, neutralizing activity in plasma decreases by fivefold in pseudotype virus assays. By contrast, the number of RBD-specific memory B cells remains unchanged at 6.2 months after infection. Memory B cells display clonal turnover after 6.2 months, and the antibodies that they express have greater somatic hypermutation, resistance to RBD mutations and increased potency, indicative of continued evolution of the humoral response. Immunofluorescence and PCR analyses of intestinal biopsies obtained from asymptomatic individuals at 4 months after the onset of coronavirus disease 2019 (COVID-19) revealed the persistence of SARS-CoV-2 nucleic acids and immunoreactivity in the small bowel of 7 out of 14 individuals. We conclude that the memory B cell response to SARS-CoV-2 evolves between 1.3 and 6.2 months after infection in a manner that is consistent with antigen persistence. In a cohort of 87 individuals with COVID-19, the memory B cell response at 6.2 months after the onset of disease evolves in a manner that is consistent with the persistence of SARS-CoV-2 antigen.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/1 / 13/31 / 13/51 / 14/34 / 49 / 631/250/2152/2153 / 631/250/2499 / 631/326/596/4130 / Adolescent / Adult / Aged / Analysis / Antibodies / Antibodies, Monoclonal - blood / Antibodies, Monoclonal - immunology / Antibodies, Neutralizing - blood / Antibodies, Neutralizing - genetics / Antibodies, Neutralizing - immunology / Antibodies, Viral - blood / Antibodies, Viral - genetics / Antibodies, Viral - immunology / Antigens / Antigens, Viral - chemistry / Antigens, Viral - genetics / Antigens, Viral - immunology / B-Lymphocytes - cytology / B-Lymphocytes - immunology / Biopsy / Cloning / Cohort Studies / Coronaviridae / Coronaviruses / COVID-19 / COVID-19 - blood / COVID-19 - immunology / Evolution / Flow cytometry / Fluorescent Antibody Technique / Humanities and Social Sciences / Humans / Immune response (humoral) / Immunity (Disease) / Immunity, Humoral - genetics / Immunity, Humoral - immunology / Immunofluorescence / Immunoglobulin A / Immunoglobulin A - immunology / Immunoglobulin G / Immunoglobulin G - immunology / Immunoglobulin M - immunology / Immunologic Memory - immunology / Immunological memory / Immunoreactivity / Infections / Intestines - immunology / Lymphocytes B / Memory cells / Middle Aged / multidisciplinary / Mutation / Natural history / Neutralizing / Nucleic acids / Plasma / Respiratory diseases / SARS-CoV-2 - immunology / Science / Science (multidisciplinary) / Severe acute respiratory syndrome coronavirus 2 / Small intestine / Somatic hypermutation / Somatic Hypermutation, Immunoglobulin / Spike Glycoprotein, Coronavirus - chemistry / Spike Glycoprotein, Coronavirus - genetics / Spike Glycoprotein, Coronavirus - immunology / Spike protein / Time Factors / Viral antibodies / Viral diseases / Young Adult