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Diverse activation of microglia by chemokine (C-C motif) ligand 2 overexpression in brain
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Diverse activation of microglia by chemokine (C-C motif) ligand 2 overexpression in brain
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Journal Article
Diverse activation of microglia by chemokine (C-C motif) ligand 2 overexpression in brain
2013
Overview
Background
The chemokine (C-C motif) ligand 2 (CCL2) is a monocyte chemoattractant protein that mediates macrophage recruitment and migration during peripheral and central nervous system (CNS) inflammation.
Methods
To determine the impact of CCL2 in inflammation
in vivo
and to elucidate the CCL2-induced polarization of activated brain microglia, we delivered CCL2 into the brains of wild-type mice via recombinant adeno-associated virus serotype 9 (rAAV-9) driven by the chicken β-actin promoter. We measured microglial activation using histological and chemical measurement and recruitment of monocytes using histology and flow cytometry.
Results
The overexpression of CCL2 in the CNS induced significant activation of brain resident microglia. CD45 and major histocompatibility complex class II immunoreactivity significantly increased at the sites of CCL2 administration. Histological characterization of the microglial phenotype revealed the elevation of “classically activated” microglial markers, such as calgranulin B and IL-1β, as well as markers associated with “alternative activation” of microglia, including YM1 and arginase 1. The protein expression profile in the hippocampus demonstrated markedly increased levels of IL-6, GM-CSF and eotaxin (CCL-11) in response to CCL2, but no changes in the levels of other cytokines, including TNF-α and IFN-γ. Moreover, real-time PCR analysis confirmed increases in mRNA levels of gene transcripts associated with neuroinflammation following CCL2 overexpression. Finally, we investigated the chemotactic properties of CCL2
in vivo
by performing adoptive transfer of bone marrow–derived cells (BMDCs) isolated from donor mice that ubiquitously expressed green fluorescent protein. Flow cytometry and histological analyses indicated that BMDCs extravasated into brain parenchyma and colabeled with microglial markers.
Conclusion
Taken together, our results suggest that CCL2 strongly activates resident microglia in the brain. Both pro- and anti-inflammatory activation of microglia were prominent, with no bias toward the M1 or M2 phenotype in the activated cells. As expected, CCL2 overexpression actively recruited circulating monocytes into the CNS. Thus, CCL2 expression in mouse brain induces microglial activation and represents an efficient method for recruitment of peripheral macrophages.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V
Subject
/ Animals
/ Biomedical and Life Sciences
/ Bone Marrow Cells - drug effects
/ Brain Chemistry - physiology
/ Cell Movement - drug effects
/ Chemokine CCL2 - biosynthesis
/ Disease
/ Gene Expression - drug effects
/ Humans
/ Image Processing, Computer-Assisted
/ Ligands
/ Macrophage Activation - drug effects
/ Mice
/ Proteins
/ Real-Time Polymerase Chain Reaction
/ Signal Transduction - drug effects
/ Studies
