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Functional analysis of alcohol dehydrogenase (ADH) genes in Pichia pastoris
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Functional analysis of alcohol dehydrogenase (ADH) genes in Pichia pastoris
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Journal Article
Functional analysis of alcohol dehydrogenase (ADH) genes in Pichia pastoris
2016
Overview
OBJECTIVES: To characterize the genes responsible for ethanol utilization in Pichia pastoris. RESULTS: ADH3 (XM_002491337) and ADH (FN392323) genes were disrupted in P. pastoris. The ADH3 mutant strain, MK115 (Δadh3), lost its ability to grow on minimal ethanol media but produced ethanol in minimal glucose medium. ADH3p was responsible for 92 % of total Adh enzyme activity in glucose media. The double knockout strain MK117 (Δadh3Δadh) also produced ethanol. The Adh activities of X33 and MK116 (Δadh) strains were not different. Thus, the ADH gene does not play a role in ethanol metabolism. CONCLUSION: The PpADH3 is the only gene responsible for consumption of ethanol in P. pastoris.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
