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CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions
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CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions
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Journal Article
CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions
2021
Overview
Vertebrate genomes are partitioned into contact domains defined by enhanced internal contact frequency and formed by two principal mechanisms: compartmentalization of transcriptionally active and inactive domains, and stalling of chromosomal loop-extruding cohesin by CTCF bound at domain boundaries. While
Drosophila
has widespread contact domains and CTCF, it is currently unclear whether CTCF-dependent domains exist in flies. We genetically ablate
CTCF
in
Drosophila
and examine impacts on genome folding and transcriptional regulation in the central nervous system. We find that CTCF is required to form a small fraction of all domain boundaries, while critically controlling expression patterns of certain genes and supporting nervous system function. We also find that CTCF recruits the pervasive boundary-associated factor Cp190 to CTCF-occupied boundaries and co-regulates a subset of genes near boundaries together with Cp190. These results highlight a profound difference in CTCF-requirement for genome folding in flies and vertebrates, in which a large fraction of boundaries are CTCF-dependent and suggest that CTCF has played mutable roles in genome architecture and direct gene expression control during metazoan evolution.
Although the Drosophila genome has widespread contact domains and CTCF, it remains unclear whether CTCF-dependent domains exist in flies. Here, the authors ablate CTCF in Drosophila and find that CTCF is required to form a small fraction of all domain boundaries, suggesting differences in the role of CTCF for genome folding in flies and vertebrates.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38
/ 38/15
/ 38/91
/ 42
/ 45
/ 45/41
/ 631/136
/ 64
/ 64/24
/ Ablation
/ Animals
/ CCCTC-Binding Factor - genetics
/ CCCTC-Binding Factor - metabolism
/ Cohesin
/ Domains
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Female
/ Flies
/ Folding
/ Genes
/ Genome
/ Genomes
/ Humanities and Social Sciences
/ Insects
/ Male
/ Microtubule-Associated Proteins - metabolism
/ Science
/ Stalling
