Journal Article

First assessment of plague in terrestrial small mammals and fleas from Makira Natural Park and surroundings, North-eastern Madagascar

Rasoamalala, Fanohinjanaharinirina, Jori, Ferran, Linchant, Julie, Guis, Hélène, Harimalala, Mireille, Rajerison, Minoarisoa, Kassié, Daouda, Walter, Pierre, Raritahiry, Sylvie Claudia, Ramasindrazana, Beza, Maminirina, Lanto Andrianarijaona, Keatts, Lucy, Michaux, Johan, Martin, Cynthia Haingotiana

2025

Overview

Plague, a zoonosis caused by Yersinia pestis, is endemic in Madagascar but knowledge on the epidemiological situation in the northern focus remains unclear. The aim of this study was to investigate the circulation of Y. pestis in terrestrial small mammals in north eastern Madagascar, where suspected plague outbreaks have been reported. Sampling of terrestrial small mammals and their fleas was carried out in 22 trapping sites within 9 localities of the two sectors (1 and 3) of Makira Natural Park (MNP) and surroundings, from 2020 to 2022. Yersinia pestis was investigated in terrestrial small mammal spleen samples and their fleas using bacteriological, serological and molecular methods. A total of 614 terrestrial small mammals composed of eight species and 1,754 individual fleas were collected following 4,880 trap-nights. The black rat (Rattus rattus) represented the majority (87.8%) of the small mammal species caught. Flea infestation rate was higher in sector 3 compared to sector 1. In sector 3, Xenopsylla brasiliensis, a plague vector, represented 66.4% of fleas identified. Further, one plague seropositive R. rattus individual, captured inside a house, and one Ctenocephalides felis specimen, collected on another R. rattus, was positive on PCR in this sector. Despite low detection rates, we confirmed the circulation of Y. pestis in our study area (one rat seropositive and one flea PCR positive) and highlight the risk of potential human transmission. Our results also suggest that R. rattus contributes to the maintenance and transmission of plague in MNP, as described for other areas in Madagascar. Further, these findings contribute to documentation of the known geographic distribution of the endemic plague vector S. fonquerniei and X. brasiliensis. The confirmation of the circulation of the Y. pestis through serological and molecular diagnostics in small mammals and fleas underscores the urgent need to assess awareness levels of risk factors and symptoms to monitor among local communities and health workers and ensure that trained rapid response teams are prepared to intervene promptly upon suspect case detection. The risk and epidemiology of plague circulation in remote rural areas of Madagascar remains insufficiently studied. Addressing this gap is crucial, as a more comprehensive understanding of the distribution and dynamics of the wild animal hosts, their vectors and host-vector interactions will enhance risk assessment and prevention for plague emergence and improve mitigation and early control of potential outbreaks.

• Publisher: Public Library of Science,Public Library of Science (PLoS),PLOS
• Subject: Animals / Biology and Life Sciences / Causes of / Disease transmission / Distribution / Epidemiology / Female / Flea Infestations / Flea Infestations - epidemiology / Flea Infestations - veterinary / Fleas / France / Health aspects / Humans / Infectious Diseases / Life Sciences / Madagascar / Madagascar - epidemiology / Male / Mammals / Mammals - microbiology / Medical research / Medicine and Health Sciences / Medicine, Experimental / Natural resources / Parks / People and Places / Plague / Plague - epidemiology / Plague - microbiology / Plague - veterinary / Public Health, Environmental and Occupational Health / Rats / Risk factors / Sciences du vivant / Siphonaptera / Siphonaptera - microbiology / Yersinia pestis / Yersinia pestis - genetics / Yersinia pestis - isolation & purification / Zoologie / Zoology / Zoonoses