Journal Article

Alternative empirical Bayes models for adjusting for batch effects in genomic studies

Manimaran, Solaiappan, Jenkins, David F., Zhang, Yuqing, Johnson, W. Evan

2018

Overview

Background Combining genomic data sets from multiple studies is advantageous to increase statistical power in studies where logistical considerations restrict sample size or require the sequential generation of data. However, significant technical heterogeneity is commonly observed across multiple batches of data that are generated from different processing or reagent batches, experimenters, protocols, or profiling platforms. These so-called batch effects often confound true biological relationships in the data, reducing the power benefits of combining multiple batches, and may even lead to spurious results in some combined studies. Therefore there is significant need for effective methods and software tools that account for batch effects in high-throughput genomic studies. Results Here we contribute multiple methods and software tools for improved combination and analysis of data from multiple batches. In particular, we provide batch effect solutions for cases where the severity of the batch effects is not extreme, and for cases where one high-quality batch can serve as a reference, such as the training set in a biomarker study. We illustrate our approaches and software in both simulated and real data scenarios. Conclusions We demonstrate the value of these new contributions compared to currently established approaches in the specified batch correction situations.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Algorithms / Batch effects / Batch processing / Bayes Theorem / Bayesian analysis / Bias / Bioinformatics / Biomarker development / Biomarkers / Biomedical and Life Sciences / Comparative analysis / Computational Biology/Bioinformatics / Computer Appl. in Life Sciences / Computer programs / Computer simulation / Data integration / Data processing / Decomposition / DNA methylation / Empirical analysis / Empirical Bayes models / Experiments / Gene expression / Genome-wide association studies / Genomes / Genomics / Genomics - methods / Heterogeneity / Humans / Life Sciences / Lung cancer / Methodology / Methodology Article / Microarrays / Reagents / Research Design / Researchers / Software / Software development tools / Studies / Transcriptome analysis