Journal Article

Ambient fine particulate matter inhibits 15-lipoxygenases to promote lung carcinogenesis

Underwood, Malcolm J., Li, Wende, Ho, Rocky L. K., Wang, Shanshan, Gali, Nirmal Kumar, Jia, Hao, Wan, Innes Y. P., Long, Xiang, Mok, Tony S. K., Yang, Shucai, Ning, Zhi, Li, Ming-Yue, Ng, Calvin S. H., Kong, Angel W. Y., Liu, Yi, Zhao, Zhili, Chen, George G., Chak, Ernest C. W., Du, Jing, Liu, Li-Zhong, Qi, Haolong

2019

Overview

Background Epidemiological observations have demonstrated that ambient fine particulate matter with d p  < 2.5 μm (PM 2.5 ) as the major factor responsible for the increasing incidence of lung cancer in never-smokers. However, there are very limited experimental data to support the association of PM 2.5 with lung carcinogenesis and to compare PM 2.5 with smoking carcinogens. Methods To study whether PM 2.5 can contribute to lung tumorigenesis in a way similar to smoking carcinogen 4-methylnitrosamino-l-3-pyridyl-butanone (NNK) via 15-lipoxygenases (15-LOXs) reduction, normal lung epithelial cells and cancer cells were treated with NNK or PM 2.5 and then epigenetically and post-translationally examined the cellular and molecular profiles of the cells. The data were verified in lung cancer samples and a mouse lung tumor model. Results We found that similar to smoking carcinogen NNK, PM2.5 significantly enhanced cell proliferation, migration and invasion, but reduced the levels of 15-lipoxygenases-1 (15-LOX1) and 15-lipoxygenases-2 (15-LOX2), both of which were also obviously decreased in lung cancer tissues. 15-LOX1/15-LOX2 overexpression inhibited the oncogenic cell functions induced by PM2.5/NNK. The tumor formation and growth were significantly higher/faster in mice implanted with PM2.5- or NNK-treated NCI-H23 cells, accompanied with a reduction of 15-LOX1/15-LOX2. Moreover, 15-LOX1 expression was epigenetically regulated at methylation level by PM2.5/NNK, while both 15-LOX1 and 15-LOX2 could be significantly inhibited by a set of PM2.5/NNK-mediated microRNAs. Conclusion Collectively, PM2.5 can function as the smoking carcinogen NNK to induce lung tumorigenesis by inhibiting 15-LOX1/15-LOX2.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: 15-lipoxygenases (15-LOXs) / Air pollution / Animals / Apoptosis / Arachidonate 15-Lipoxygenase - chemistry / Biomarkers, Tumor - metabolism / Biomedical and Life Sciences / Biomedicine / Cancer cells / Cancer Research / Carcinogenesis / Carcinogenesis - chemically induced / Carcinogenesis - metabolism / Carcinogenesis - pathology / Care and treatment / Case-Control Studies / Cell Movement / Cell Proliferation / Development and progression / Epigenetic and post-translational regulation / Epigenetic inheritance / Female / Gene Expression Regulation, Neoplastic / Genetic aspects / Health aspects / Humans / Immunology / Lipoxygenase Inhibitors - adverse effects / Lung cancer / Lung Neoplasms - enzymology / Lung Neoplasms - etiology / Lung Neoplasms - pathology / Methylation / Mice / Mice, Inbred BALB C / Mice, Nude / MicroRNA / Nitrosamines - toxicity / NNK / Oncology / Particulate Matter - adverse effects / PM2.5 / Prognosis / Risk factors / Smokers / Smoking / Smoking - adverse effects / Tumor Cells, Cultured / Tumors / Xenograft Model Antitumor Assays