Journal Article

Withaferin A inhibits Chikungunya virus nsP2 protease and shows antiviral activity in the cell culture and mouse model of virus infection

Srivastava, Mitul, Bharadwaj, Kanchan, Sharma, Kiran Bala, Sharma, Ghanshyam, Deb, Arundhati, Subramani, Chandru, Wadhwa, Renu, Ganesh, Khashpatika, Sahoo, Pankaj Kumar, Sharma, Anshula, KA, Shouri, Sehrawat, Sapna, Saurav, Suman, Motiani, Rajender K., Singh, Ramandeep, Asthana, Shailendra, Basu, Brohmomoy, Rout, Archana, Vrati, Sudhanshu, Chugh, Saurabh, Jain, Deepti, Balyan, Shivani

2024

Overview

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus causing fever, myalgia, and debilitating joint swelling and pain, which in many patients becomes chronic. The frequent epidemics of CHIKV across the world pose a significant public health burden necessitating the development of effective antiviral therapeutics. A cellular imaging-based high-content screening of natural compounds identified withaferin A (WFA), a steroidal lactone isolated from the plant Withania somnifera , as a potent antiviral against CHIKV. In the ERMS cells, WFA inhibited CHIKV replication early during the life cycle by binding the CHIKV non-structural protein nsP2 and inhibiting its protease activity. This inhibited the viral polyprotein processing and the minus-sense viral RNA synthesis. WFA mounted the nsP2 protease inhibitory activity through its oxidising property as the reducing agents N-acetylcysteine and Glutathione-monoethyl ester effectively reversed the WFA-mediated protease inhibition in vitro and abolished the WFA-mediated antiviral activity in cultured cells. WFA inhibited CHIKV replication in the C57BL/6 mouse model of chikungunya disease, resulting in significantly lower viremia. Importantly, CHIKV-infected mice showed significant joint swelling which was not seen in WFA-treated mice. These data demonstrate the potential of WFA as a novel CHIKV antiviral.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acetylcysteine / Animals / Antiviral activity / Antiviral agents / Antiviral Agents - pharmacology / Cell culture / Chikungunya fever / Chikungunya Fever - drug therapy / Chikungunya Fever - virology / Chikungunya virus / Chikungunya virus - drug effects / Cloning / Cysteine Endopeptidases - metabolism / Disease Models, Animal / Disease transmission / Drug development / Drug therapy / Experiments / Genomes / Glutathione / Health aspects / Humans / Infections / Laboratory animals / Materia medica, Vegetable / Mice / Mice, Inbred C57BL / Myalgia / Pharmaceutical research / Plant extracts / Protease / Protease inhibitors / Proteins / Public health / Reducing agents / Replication / RNA polymerase / Swelling / Transcription / Vector-borne diseases / Viral Nonstructural Proteins - antagonists & inhibitors / Viral Nonstructural Proteins - metabolism / Viremia / Virus Replication - drug effects / Viruses / Withanolides - pharmacology