Journal Article

Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases

Pap, Domonkos, Cseh, Áron, Szebeni, Beáta, Ónody, Anna, Veres, Gábor, Oswald, Franz, Vannay, Ádám, Szabó, Attila J., Rokonay, Réka, Sziksz, Erna, Veres-Székely, Apor

2021

Overview

Background Recently, increased interleukin (IL)-24 expression has been demonstrated in the colon biopsies of adult patients with inflammatory bowel disease (IBD). However, the role of IL-24 in the pathomechanism of IBD is still largely unknown. Methods Presence of IL-24 was determined in the samples of children with IBD and in the colon of dextran sodium sulfate (DSS) treated mice. Effect of inflammatory factors on IL24 expression was determined in peripheral blood (PBMCs) and lamina propria mononuclear cells (LPMCs). Also, the impact of IL-24 was investigated on HT-29 epithelial cells and CCD-18Co colon fibroblasts. Expression of tissue remodeling related genes was investigated in the colon of wild type (WT) mice locally treated with IL-24 and in the colon of DSS treated WT and Il20rb knock out (KO) mice. Results  Increased amount of IL-24 was demonstrated in the serum and colon samples of children with IBD and DSS treated mice compared to that of controls. IL-1β, LPS or H 2 O 2 treatment increased the expression of IL24 in PBMCs and LPMCs. IL-24 treatment resulted in increased amount of TGF-β and PDGF-B in HT-29 cells and enhanced the expression of extracellular matrix (ECM)-related genes and the motility of CCD-18Co cells. Similarly, local IL-24 treatment increased the colonic Tgfb1 and Pdgfb expression of WT mice. Moreover, expression of pro-fibrotic Tgfb1 and Pdgfb were lower in the colon of DSS treated Il20rb KO compared to that of WT mice. The disease activity index of colitis was less severe in DSS treated Il20rb KO compared to WT mice. Conclusion Our study suggest that IL-24 may play a significant role in the mucosal remodeling of patients with IBD by promoting pro-fibrotic processes.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abdomen / Animals / Biomedical and Life Sciences / Biomedicine / Biopsy / Cell culture / Children / Colitis / Colon / Cytokines / Development and progression / Dextran / Dextran Sulfate / Disease / Disease Models, Animal / Drinking water / Epithelial cells / Experiments / Extracellular matrix / Fibroblasts / Fibrosis / Health aspects / Humans / Hydrogen Peroxide / IBD / IL-1β / IL-24 / Immunobiology and immunotherapy / Inflammatory bowel disease / Inflammatory Bowel Diseases / Interleukin / Interleukin 24 / Interleukins / Intestinal mucosa / Intestine / Lamina propria / Leukocytes (mononuclear) / Lipopolysaccharides / Medicine/Public Health / Mice / Mice, Inbred C57BL / Mucosa / Mucous Membrane / Pediatrics / Peripheral blood / Physiological aspects / Platelet-derived growth factor / Sodium sulfate / Tissue remodeling / Transforming growth factor-b1