Journal Article

Wnt5a-mediated autophagy contributes to the epithelial-mesenchymal transition of human bronchial epithelial cells during asthma

Liu, Shao-Kun, Zhang, Chen-Yu, Yang, Jin-Tong, Jin, Ling, Liu, Yu-Biao, Tan, Xiao-Hua, Xiong, Jian-Bing, Guan, Cha-Xiang, Zhou, Yong, Yang, Nan-Shi-Yu, Yang, Hui-Hui

2024

Overview

Background The epithelial-mesenchymal transition (EMT) of human bronchial epithelial cells (HBECs) is essential for airway remodeling during asthma. Wnt5a has been implicated in various lung diseases, while its role in the EMT of HBECs during asthma is yet to be determined. This study sought to define whether Wnt5a initiated EMT, leading to airway remodeling through the induction of autophagy in HBECs. Methods Microarray analysis was used to investigate the expression change of WNT5A in asthma patients. In parallel, EMT models were induced using 16HBE cells by exposing them to house dust mites (HDM) or interleukin-4 (IL-4), and then the expression of Wnt5a was observed. Using in vitro gain- and loss-of-function approaches via Wnt5a mimic peptide FOXY5 and Wnt5a inhibitor BOX5, the alterations in the expression of the epithelial marker E-cadherin and the mesenchymal marker protein were observed. Mechanistically, the Ca 2+ /CaMKII signaling pathway and autophagy were evaluated. An autophagy inhibitor 3-MA was used to examine Wnt5a in the regulation of autophagy during EMT. Furthermore, we used a CaMKII inhibitor KN-93 to determine whether Wnt5a induced autophagy overactivation and EMT via the Ca 2+ /CaMKII signaling pathway. Results Asthma patients exhibited a significant increase in the gene expression of WNT5A compared to the healthy control. Upon HDM and IL-4 treatments, we observed that Wnt5a gene and protein expression levels were significantly increased in 16HBE cells. Interestingly, Wnt5a mimic peptide FOXY5 significantly inhibited E-cadherin and upregulated α-SMA, Collagen I, and autophagy marker proteins (Beclin1 and LC3-II). Rhodamine-phalloidin staining showed that FOXY5 resulted in a rearrangement of the cytoskeleton and an increase in the quantity of stress fibers in 16HBE cells. Importantly, blocking Wnt5a with BOX5 significantly inhibited autophagy and EMT induced by IL-4 in 16HBE cells. Mechanistically, autophagy inhibitor 3-MA and CaMKII inhibitor KN-93 reduced the EMT of 16HBE cells caused by FOXY5, as well as the increase in stress fibers, cell adhesion, and autophagy. Conclusion This study illustrates a new link in the Wnt5a-Ca 2+ /CaMKII-autophagy axis to triggering airway remodeling. Our findings may provide novel strategies for the treatment of EMT-related diseases.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Adult / Airway remodeling / Antibodies / Asthma / Asthma - genetics / Asthma - metabolism / Asthma - pathology / Autophagy / Biomedical and Life Sciences / Biomedicine / Bronchi - metabolism / Bronchi - pathology / Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism / Calmodulin-dependent kinase II / Cell culture / Cell Line / Disease / Epithelial Cells - metabolism / Epithelial-Mesenchymal Transition / Extracellular matrix / Female / Fibroblasts / Growth factors / Humans / Kinases / Male / Melanoma / Metastasis / Middle Aged / Molecular Medicine / Peptides / Proteins / Research Article / Signal Transduction / Smooth muscle / Wnt-5a Protein - genetics / Wnt-5a Protein - metabolism / Wnt5a