Journal Article

Hyperbaric oxygen reduces inflammation, oxygenates injured muscle, and regenerates skeletal muscle via macrophage and satellite cell activation

Yamamoto, Naoki, Tsuji, Kunikazu, Sekiya, Ichiro, Yagishita, Kazuyoshi, Enomoto, Mitsuhiro, Muneta, Takeshi, Oyaizu, Takuya, Okawa, Atsushi, Horie, Masaki

2018

Overview

Hyperbaric oxygen treatment (HBO) promotes rapid recovery from soft tissue injuries. However, the healing mechanism is unclear. Here we assessed the effects of HBO on contused calf muscles in a rat skeletal muscle injury model. An experimental HBO chamber was developed and rats were treated with 100% oxygen, 2.5 atmospheres absolute for 2 h/day after injury. HBO reduced early lower limb volume and muscle wet weight in contused muscles, and promoted muscle isometric strength 7 days after injury. HBO suppressed the elevation of circulating macrophages in the acute phase and then accelerated macrophage invasion into the contused muscle. This environment also increased the number of proliferating and differentiating satellite cells and the amount of regenerated muscle fibers. In the early phase after injury, HBO stimulated the IL-6/STAT3 pathway in contused muscles. Our results demonstrate that HBO has a dual role in decreasing inflammation and accelerating myogenesis in muscle contusion injuries.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/105 / 13/21 / 13/31 / 13/51 / 14/63 / 692/698/1671/1668/1973 / 692/699/578 / Animals / Cell activation / Cell Differentiation - drug effects / Cell Proliferation - drug effects / Gene Expression Regulation / Humanities and Social Sciences / Hyperbaric oxygen / Hyperbaric Oxygenation - methods / Inflammation / Injuries / Interleukin 6 / Interleukin-6 - genetics / Interleukin-6 - metabolism / Isometric Contraction - drug effects / Isometric Contraction - physiology / Macrophages / Macrophages - cytology / Macrophages - drug effects / Macrophages - metabolism / Male / multidisciplinary / Muscle contraction / Muscle Development - drug effects / Muscle Development - genetics / Muscle, Skeletal - drug effects / Muscle, Skeletal - injuries / Muscle, Skeletal - metabolism / Muscles / Musculoskeletal system / Myogenesis / Oxygen / Oxygen - pharmacology / Rats / Rats, Wistar / Recovery of Function - drug effects / Recovery of Function - physiology / Regeneration - drug effects / Rodents / Satellite cells / Satellite Cells, Skeletal Muscle - cytology / Satellite Cells, Skeletal Muscle - drug effects / Satellite Cells, Skeletal Muscle - metabolism / Science / Science (multidisciplinary) / Signal Transduction / Skeletal muscle / Soft Tissue Injuries - genetics / Soft Tissue Injuries - metabolism / Soft Tissue Injuries - pathology / Soft Tissue Injuries - therapy / Stat3 protein / STAT3 Transcription Factor - genetics / STAT3 Transcription Factor - metabolism