Journal Article

Melanoma cell-secreted exosomal miR-155-5p induce proangiogenic switch of cancer-associated fibroblasts via SOCS1/JAK2/STAT3 signaling pathway

Yan, Tinglin, Zhou, Xiaocheng, Wang, Hui, Chen, Yang, Huang, Chunming, Wang, Lin, Jiang, Erhui, Shao, Zhe, Xu, Zhi, Liu, Ke, Shang, Zhengjun

2018

Overview

Background Cancer-associated fibroblasts (CAFs) have been widely reported to promote tumor angiogenesis. However, the underlying mechanisms of the proangiogenic switch of CAFs remain poorly understood. This study aims to clarify the mechanisms underlying the proangiogenic switch of CAFs. Methods NIH/3T3 cells were treated with B16 and B16F10-derived exosomes. Then the CAFs markers and proangiogenic factors were detected by RT-PCR and Western blot. CCK-8 assay, transwell migration assay, tube formation assay, and in vivo Matrigel plug assay were conducted to determine the proangiogenic capability of CAFs. Western blot and AG490 were used to investigate the role of Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in the proangiogenic switch of CAFs. Bioinformatics analysis, luciferase reporter assay, microRNA mimic and inhibitor, and xenograft models were used to investigate the role of mmu-miR-155-5p (miR-155) in the proangiogenic switch of CAFs. Results In this study, we show that melanoma cell-secreted exosomes can induce reprogramming of fibroblasts into CAFs and that exosomal miR-155 can trigger the proangiogenic switch of CAFs. Mechanistically exosomal miR-155 can be delivered into fibroblasts and promote the expression of proangiogenic factors, including vascular endothelial growth factor A (VEGFa), fibroblast growth factor 2 (FGF2), and matrix metalloproteinase 9 (MMP9), by directly targetin g suppressor of cytokine signaling 1 (SOCS1) . Downregulation of SOCS1 activates JAK2/STAT3 signaling pathway and elevates the expression levels of VEGFa, FGF2, and MMP9 in fibroblasts. Treatment with exosomes containing overexpressed miR-155 can promote angiogenesis, and the reduction of miR-155 in melanoma cell-secreted exosomes alleviates angiogenesis in vitro and in vivo. Conclusions These results demonstrate that by promoting the expression of proangiogenic factors in recipient fibroblasts via SOCS1/JAK2/STAT3 signaling pathway, melanoma cell-secreted exosomal miR-155 can induce the proangiogenic switch of CAFs. Although tumor angiogenesis is modulated by various factors, exosomal miR-155 may be a potential target for controlling melanoma angiogenesis and used to set up novel strategies to treat melanoma.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Angiogenesis / Animals / Apoptosis / Biomedical and Life Sciences / Biomedicine / Cancer / Cancer Research / Cancer-associated fibroblasts / Cancer-Associated Fibroblasts - metabolism / Cancer-Associated Fibroblasts - pathology / Cell adhesion & migration / Cell growth / Cell Proliferation - physiology / Cytokines / Enzymes / Exosomes / Exosomes - genetics / Exosomes - metabolism / Fibroblasts / Gene expression / Immunology / Investigations / JAK2/STAT3 signaling pathway / Janus Kinase 2 - metabolism / Kinases / Male / Melanoma / Melanoma, Experimental - blood supply / Melanoma, Experimental - genetics / Melanoma, Experimental - metabolism / Melanoma, Experimental - pathology / Metastasis / Mice / Mice, Inbred C57BL / MicroRNA / MicroRNAs / MicroRNAs - genetics / MicroRNAs - metabolism / Mmu-miR-155-5p / Neovascularization / Neovascularization, Pathologic - genetics / Neovascularization, Pathologic - metabolism / Neovascularization, Pathologic - pathology / NIH 3T3 Cells / Oncology / Physiological aspects / Proteins / Signal Transduction / STAT3 Transcription Factor - metabolism / Suppressor of Cytokine Signaling 1 Protein - metabolism / Transfection / Tumors / Vascular endothelial growth factor