Asset Details
Do you wish to reserve the book?
Detection of circulating tumor cells in patients with lung cancer using a rare cell sorter: a pilot study
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Detection of circulating tumor cells in patients with lung cancer using a rare cell sorter: a pilot study
Do you wish to request the book?
Detection of circulating tumor cells in patients with lung cancer using a rare cell sorter: a pilot study
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Detection of circulating tumor cells in patients with lung cancer using a rare cell sorter: a pilot study
2024
Overview
Background
We developed a Rare Cell Sorter (RCS) for collecting single cell including circulating tumor cells (CTCs). This single-institution pilot study evaluated the ability of this device to detect tumor-like cells in patients with lung cancer and confirmed their genuineness based on the epidermal growth factor receptor (EGFR) mutation concordance with tissue samples.
Methods
This study included patients treated for lung cancer from September 2021 to August 2022 in University of Tsukuba Hospital. Peripheral blood samples were obtained before surgery or during periodic medical checks for patients treated with drugs. We used the RCS to capture cells based on size. The cells were stained, and the Hoechst-positive, CD45-negative, and epithelial celladhesion molecule (EpCAM)- positive cells were defined as CTCs, were collected. The presumptive CTCs were counted and tested using digital droplet polymerase chain reaction for EGFR mutations and compared with the tissue EGFR status to check concordance.
Results
Eighteen patients were included in this study and CTCs were detected in 6 patients (33%). The CTCs from three patients showed EGFR mutation, and the EGFR mutation status of CTCs concorded with that of tissue samples in 83% of the cases (5/6). Only one CTC showed a different status from the tissue, and the concordance rate of EGFR status between CTCs and the tissue was 96% (24/25).
Conclusion
The ability of the RCS to detect CTCs in patients with lung cancer was demonstrated based on the concordance of EGFR status in this pilot study. This novel hybrid method of CTC recovery using the RCS has the potential to recover a wide range of CTCs regardless of EpCAM. Further validation through a large-scale study is needed.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Biomarkers, Tumor - genetics
/ Biomedical and Life Sciences
/ Biopsy
/ Blood
/ Cancer
/ Epidermal growth factor receptor
/ Epidermal growth factor receptors
/ Epithelial Cell Adhesion Molecule - genetics
/ Epithelial Cell Adhesion Molecule - metabolism
/ Epithelial-mesenchymal transition
/ Ethylenediaminetetraacetic acid
/ Female
/ Health Promotion and Disease Prevention
/ Humans
/ Kinases
/ Male
/ Methods
/ Mutation
/ Neoplastic Cells, Circulating - metabolism
/ Neoplastic Cells, Circulating - pathology
/ Oncology
/ Patients
/ Tumors
