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Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
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Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
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Journal Article
Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
2020
Overview
To characterize the molecular mechanisms underlying life-stage transitions in
Phytophthora infestans
, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from soil in Japan. Of the more than 700 extracts, one consistently inhibited
Ph. infestans
cyst germination. Purification and identification of the active compound by ESI–MS,
1
H-NMR, and
13
C-NMR identified β-rubromycin as the inhibitor of cyst germination (IC
50
= 19.8 μg/L); β-rubromycin did not inhibit growth on rye media, sporangium formation, zoospore release, cyst formation, or appressorium formation in
Ph. infestans
. Further analyses revealed that β-rubromycin inhibited the germination of cysts and oospores in
Pythium aphanidermatum
. A chemical genetic approach revealed that β-rubromycin stimulated the expression of RIO kinase-like gene (PITG_04584) by 60-fold in
Ph. infestans
. Genetic analyses revealed that PITG_04584, which lacks close non-oomycete relatives, was involved in zoosporogenesis, cyst germination, and appressorium formation in
Ph. infestans
. These data imply that further functional analyses of PITG_04584 may contribute to new methods to suppress diseases caused by oomycetes.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 631/326
/ 631/337
/ Amino Acid Sequence - genetics
/ Cysts
/ Genetics
/ Humanities and Social Sciences
/ NMR
/ Oospores
/ Phytophthora infestans - genetics
/ Phytophthora infestans - growth & development
/ Plant Diseases - microbiology
/ Protein-Serine-Threonine Kinases - antagonists & inhibitors
/ Protein-Serine-Threonine Kinases - genetics
/ Science
