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A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues
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A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues
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Journal Article
A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues
2022
Overview
Proteolysis-targeting chimaeras (PROTACs) as well as molecular glues such as immunomodulatory drugs (IMiDs) and indisulam are drugs that induce interactions between substrate proteins and an E3 ubiquitin ligases for targeted protein degradation. Here, we develop a workflow based on proximity-dependent biotinylation by AirID to identify drug-induced neo-substrates of the E3 ligase cereblon (CRBN). Using AirID-CRBN, we detect IMiD-dependent biotinylation of CRBN neo-substrates in vitro and identify biotinylated peptides of well-known neo-substrates by mass spectrometry with high specificity and selectivity. Additional analyses reveal ZMYM2 and ZMYM2-FGFR1 fusion protein—responsible for the 8p11 syndrome involved in acute myeloid leukaemia—as CRBN neo-substrates. Furthermore, AirID-DCAF15 and AirID-CRBN biotinylate neo-substrates targeted by indisulam and PROTACs, respectively, suggesting that this approach has the potential to serve as a general strategy for characterizing drug-inducible protein–protein interactions in cells.
PROTACs and molecular glues target E3 ubiquitin ligases to substrate proteins. Here, the authors develop a proximity biotinylation-based method to identify drug-induced E3 ligase-substrate interactions, enabling the assessment of the target spectrum of PROTACs and molecular glues in cells.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 82/58
/ Adaptor Proteins, Signal Transducing - genetics
/ Adaptor Proteins, Signal Transducing - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Drugs
/ Fibroblast growth factor receptor 1
/ Glues
/ Humanities and Social Sciences
/ Humans
/ Immunologic Factors - pharmacology
/ Intracellular Signaling Peptides and Proteins - genetics
/ Intracellular Signaling Peptides and Proteins - metabolism
/ Leukemia
/ Peptides
/ Proteins
/ Receptor, Fibroblast Growth Factor, Type 1 - genetics
/ Receptor, Fibroblast Growth Factor, Type 1 - metabolism
/ Science
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Ubiquitin-Protein Ligases - genetics
/ Ubiquitin-Protein Ligases - metabolism
/ Workflow
