Journal Article

Time of exposure and assessment influence the mortality induced by insecticides against metabolic resistant mosquitoes

Ngonyani, Safina, Muganga, Joseph B., Bradley, John, Mpelepele, Ahmadi Bakari, Bosselmann, Rune, Mboma, Zawadi Mageni, Matanila, Isaya, Skovmand, Ole, Moore, Sarah Jane, Odufuwa, Olukayode G.

2024

Overview

Background Increasing metabolic resistance in malaria vector mosquitoes resulted in the development of insecticide-treated nets (ITNs) with active ingredients (AI) that target them. Bioassays that accurately measure the mortality induced by these AIs on ITNs are needed. Mosquito metabolic enzyme expression follows a circadian rhythm. Thus, this study assessed (i) influence of the time of day of mosquito exposure and (ii) timing of assessment of mortality post exposure (24 and 72 h) to ITNs against vectors that are susceptible to pyrethroids and those with metabolic and knockdown resistance mechanisms. Methods Two cone bioassay experiments were conducted following World Health Organization (WHO) guidelines. Firstly, on ITNs incorporated with 2 g AI/kg of deltamethrin (DM) alone, or combined with 8 g AI/kg piperonyl butoxide (PBO) synergist, during the day (9:00–14:00 h) and repeated in the evening (18:00–20:00 h). This was followed by a confirmatory experiment during the afternoon (12:00–14:00 h) and repeated in the night (22:00–24:00 h) using mosquitoes unexposed or pre-exposed to PBO for 1 h before exposure to DM ITNs. Each net piece was tested with a minimum of eight cones per time (N = 24). The outcome was mortality after 24 h (M24) or 72 h (M72) of holding. Results The cone bioassays performed using metabolic resistant mosquitoes during the evening showed significantly lower M24 than those performed in the day for DM: odds ratio (OR) 0.14 [95% confidence interval (CI) 0.06–0.30, p  < 0.0001] and DM PBO [OR 0.29 (95% CI 0.18—0.49, p  < 0.0001). M72 was higher than M24 for metabolic resistant mosquitoes exposed to DM [OR 1.44 (95% CI 1.09–1.88), p  = 0.009] and DM PBO [OR 1.82 (95% CI 1.42–2.34), p  < 0.0001]. An influence of hour of experiment and time of assessment was not observed for mosquitoes that had knockdown resistance or that were pyrethroid-susceptible. Conclusions Time of day of experiment and hour of assessment of delayed mortality after exposure of mosquitoes are important considerations in evaluating insecticides that interact with mosquito metabolism to counter metabolic resistant mosquitoes. This is important when evaluating field-aged ITNs that may have lower concentrations of AI. Graphical Abstract

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Animals / Anopheles / Aquatic insects / Bio-assays / Bioassays / Biomedical and Life Sciences / Biomedicine / Circadian rhythm / Circadian rhythms / Cone bioassay / confidence interval / Control / Culicidae / Deltamethrin / Entomology / Enzymes / Evaluation / Evening / Exposure / High density polyethylenes / Human diseases / Infectious Diseases / Insecticide / insecticide application / Insecticide Resistance / Insecticide-Treated Bednets / Insecticides / Insecticides - pharmacology / Laboratories / Malaria / Medical research / Medicine, Experimental / Metabolism / Methods / Mortality / Mosquito Control - methods / Mosquito Vectors / Mosquitoes / odds ratio / Parasitology / pesticide resistance / Pesticides / Piperonyl butoxide / Piperonyl butoxide (PBO) / Piperonyl Butoxide - pharmacology / Polyesters / Product testing / Public health / pyrethrins / Pyrethrins - pharmacology / Pyrethroid / Pyrethroids / Quality standards / Resistance mechanisms / Tanzania / Testing / Time / Time of use / Tropical Medicine / Vector control / Vector-borne diseases / Vectors / Veterinary Medicine/Veterinary Science / Virology / World Health Organization