Journal Article

Effects of a new thyrotropic drug isolated from Potentilla alba on the male reproductive system of rats and offspring development

Mizina, Praskovya G., Sherwin, Catherine M., Babenko, Aleksandra N., Krepkova, Lubov V., Mkhitarov, Vladimir A., Enioutina, Elena Y., Bortnikova, Valentina V., Job, Kathleen M.

2021

Overview

Background The dysfunction of the thyroid gland is a common medical condition. Nowadays, patients frequently use medicinal herbs as complementary or alternative options to conventional drug treatments. These patients may benefit from treatment of thyroid dysfunctions with Potentilla alba L. preparations. While it has been reported that Potentilla alba preparations have low toxicity, nothing is known about their ability to affect reproductive functions in patients of childbearing age. Methods Male Wistar rats were orally treated with a thyrotrophic botanical drug, standardized Potentilla alba Dry Extract (PADE), at doses 8 and 40 times higher than the median therapeutic dose recommended for the clinical trials, for 60 consecutive days. Male Wistar rats receiving water (H 2 O) were used as controls. After completing treatment, half of the PADE-treated and control males were used to determine PADE gonadotoxicity, and the remaining half of PADE-treated and control males were mated with intact females. Two female rats were housed with one male for two estrus cycles. PADE effects on fertility and fetal/offspring development were evaluated. Results Herein, we report that oral treatment of male Wistar rats with PADE before mating with intact females instigated marked effects on male reproductive organs. Treatment significantly decreased the motility of the sperm and increased the number of pathological forms of spermatozoa. Additionally, a dose-dependent effect on Leydig cells was observed. However, these PADE effects did not significantly affect male fertility nor fetal and offspring development when PADE-treated males were mated with intact females. Conclusions PADE treatment of male rates negatively affected sperm and testicular Leydig cell morphology. However, these changes did not affect male fertility and offspring development. It is currently not known whether PADE treatment may affect human male fertility and offspring development. Therefore, these results from an animal study need to be confirmed in humans. Results from this animal study can be used to model the exposure-response relationship and adverse outcomes in humans.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Alternative medicine / Animal models / Animal reproduction / Basic research / Cell morphology / cell structures / Chiropractic Medicine / Clinical trials / complement / Complementary & Alternative Medicine / Cytology / dose response / Dose-response effects / Dose-response relationship / Drug dosages / Drug therapy / drugs / Estrus / Experiments / Females / Fertility / Fetuses / Health aspects / Health services / Herbal drug toxicity / Herbal medicine / Herbs / humans / Hyperthyroidism / Internal Medicine / Leydig cells / male fertility / Male reproductive health / Male reproductive system / Males / Medical research / Medicinal herbs / Medicinal plants / Medicine / Medicine & Public Health / Medicine, Botanic / Medicine, Herbal / Morphology / Motility / Offspring / oral administration / Organs / Patients / Potentilla alba / Preclinical drug evaluation / Pregnancy / progeny / Receiving waters / Reproductive health / Reproductive organs / Reproductive system / Research Article / Rodents / Sperm / Spermatogenesis / Spermatozoa / Thyroid / Thyroid diseases / Thyroid gland / Toxicity