Journal Article

Retinol-binding protein type 1 expression predicts poor prognosis in head and neck squamous cell carcinoma

Yu, Xin, Smeets, Ralf, Fu, Ling-ling, Friedrich, Reinhard E., Shao, Min, Yan, Ming, Feng, Hong-chao, Xu, Liya, Vollkommer, Tobias, Gosau, Martin

2024

Overview

Background Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent malignancy worldwide, with high incidence and poor survival rates. RBP1 is highly expressed in several kinds of cancer and plays a potential prognostic factor. However, the relationship between RBP1 and HNSCC were analyzed based on The Cancer Genome Atlas (TCGA) database. Materials and methods RBP1 expression and clinical information were obtained from the Cancer Genome Atlas (TCGA) database. Tumor tissue and adjacent normal tissue of 6 HNSCC patients were collected to analyze the RBP1 mRNA expression level by quantitative PCR. Cox regression analysis was used to evaluate the prognostic values of RBP1 and clinical data in HNSCC. A nomogram was also established to predict the impact of RBP1 on prognosis based on Cox multivariate results. The methylation level of RBP1 in HNSC and its prognosis were analyzed in UALACN and MethSurv. Finally, the potential biological functions of RBP1 were investigated using gene set enrichment analysis (GSEA) and single sample GSEA (ssGSEA). Results The mRNA expression levels of RBP1 were highly expressed in HNSCC tissue. The Cox analyses demonstrate that highly-expressed RBP1 is an independent prognosis marker( P  < 0.05). ROC curve analysis showed that performances of RBP1 (area under the ROC curve: 0.887, sensitivity: 84.1%, specificity: 79.9%). The methylation was increased in HNSCC patients compared with normal subjects( P  < 0.05) and was associated with better prognosis at sites cg06208339, cg12298268, cg12497564, cg15288618, cg20532370, cg23448348. Additionally, RBP1 expression is mildly associated with immune cell infiltration and immunological checkpoints. Conclusion RBP1 is overexpressed and associated with poor patient prognosis in head and neck squamous cell carcinoma.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Aged / Analysis / B cells / Bioinformatic analysis / Biomarkers, Tumor - genetics / Biomarkers, Tumor - metabolism / Biomedical and Life Sciences / Biomedicine / Cancer / Cancer Research / Cancer therapies / Care and treatment / Cell survival / Demographic aspects / Diagnosis / DNA Methylation / Female / Gene expression / Gene Expression Regulation, Neoplastic / Gene set enrichment analysis / Genetic aspects / Genomes / Genomics / Head & neck cancer / Head and neck cancer / Head and neck carcinoma / Head and Neck Neoplasms - genetics / Head and Neck Neoplasms - metabolism / Head and Neck Neoplasms - mortality / Head and Neck Neoplasms - pathology / Head and neck squamous cell carcinoma / Health aspects / Health Promotion and Disease Prevention / Humans / Lymphocytes / Male / Malignancy / Mann-Whitney U test / Medical prognosis / Medicine/Public Health / Metastases / Metastasis / Methylation / Middle Aged / Nomograms / Oncology / Prognosis / Prognosis biomarker / Protein binding / Proteins / RBP1 / Retinoids / Retinol-binding protein / Retinol-Binding Proteins, Cellular / Retinol-Binding Proteins, Plasma - genetics / Retinol-Binding Proteins, Plasma - metabolism / RNA / RNA, Messenger - genetics / RNA, Messenger - metabolism / Software / Squamous cell carcinoma / Squamous Cell Carcinoma of Head and Neck - genetics / Squamous Cell Carcinoma of Head and Neck - metabolism / Squamous Cell Carcinoma of Head and Neck - mortality / Squamous Cell Carcinoma of Head and Neck - pathology / Surgical Oncology / Survival analysis / Tumors / Vitamin A