Journal Article

Blood microbiota in HIV-infected and HIV-uninfected patients with suspected sepsis detected by metagenomic next-generation sequencing

Wu, Songjie, Zou, Shi, Liu, Jie, Song, Shihui, Chen, Qianhui, Liang, Ke, Tan, Yuting, Wang, Min, Chen, Zhong, Du, Qian

2024

Overview

Background Information on the comparison of blood microbiota between human immunodeficiency virus (HIV)-infected and HIV-uninfected patients with suspected sepsis by metagenomic next-generation sequencing (mNGS) is limited. Methods Retrospectively analysis was conducted in HIV-infected and HIV-uninfected patients with suspected sepsis at Changsha First Hospital (China) from March 2019 to August 2022. Patients who underwent blood mNGS testing were enrolled. The blood microbiota detected by mNGS were analyzed. Results A total of 233 patients with suspected sepsis who performed blood mNGS were recruited in this study, including 79 HIV-infected and 154 HIV-uninfected patients. Compared with HIV-uninfected patients, the proportions of mycobacterium ( p  = 0.001), fungus ( p  < 0.001) and viruses ( p  < 0.001) were significantly higher, while the proportion of bacteria ( p  = 0.001) was significantly lower in HIV-infected patients. The higher positive rates of non-tuberculous mycobacteriosis (NTM, p  = 0.022), Pneumocystis jirovecii ( P. jirovecii ) ( p  = 0.014), Talaromyces marneffei ( T. marneffei ) ( p  < 0.001) and cytomegalovirus (CMV) ( p  < 0.001) were observed in HIV-infected patients, compared with HIV-uninfected patients. In addition, compared with HIV-uninfected patients, the constituent ratio of T. marneffei ( p  < 0.001) in the fungus spectrum were significantly higher, while the constituent ratios of Candida ( p  < 0.001) and Aspergillus ( p  = 0.001) were significantly lower in HIV-infected patients. Conclusions Significant differences in the blood microbiota profiles exist between HIV-infected and HIV-uninfected patients with suspected sepsis.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adult / Aged / Bacteria / Bacteria - classification / Bacteria - genetics / Bacteria - isolation & purification / Blood / Blood microbiota / China / Comparative analysis / Composition / Constituents / Cytomegalovirus / Diagnosis / DNA sequencing / Female / Fungi / Genomes / Health aspects / High-Throughput Nucleotide Sequencing / HIV / HIV infection / HIV Infections - complications / HIV Infections - microbiology / Human immunodeficiency virus / Humans / Infections / Infectious Diseases / Internal Medicine / Lymphocytes / Male / Medical examination / Medical Microbiology / Medicine / Medicine & Public Health / Metagenomic next-generation sequencing / Metagenomics / Metagenomics - methods / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Middle Aged / Mycobacteriosis / Next-generation sequencing / Normal distribution / Nucleotide sequencing / Parasites / Parasitology / Pathogens / Retrospective Studies / Sepsis / Sepsis - blood / Sepsis - microbiology / Suspected sepsis / Tropical Medicine / Tuberculosis / Viruses