Journal Article

The sunflower genome provides insights into oil metabolism, flowering and Asterid evolution

Bergès, Hélène, Raftis, Frances, Hubner, Sariel, Nguyen, Thuy, Badouin, Hélène, Pouilly, Nicolas, Lelandais-Brière, Christine, Owens, Gregory L., Mandel, Jennifer R., Vear, Felicity, Claudel, Clotilde, Morien, Evan, Vandecasteele, Céline, Gouzy, Jérôme, Crespi, Martin, Kane, Nolan C., Knapp, Steven J., Sallet, Erika, Langlade, Nicolas B., Legrand, Ludovic, Schiex, Thomas, Catrice, Olivier, Fievet, Ghislain, Brunel, Dominique, Salse, Jérôme, Varès, Didier, Grassa, Christopher J., Marage, Gwenola, Pegot-Espagnet, Prune, Muños, Stéphane, Donnadieu, Cécile, Rieseberg, Loren H., Vautrin, Sonia, Lippi, Yannick, Marquand, Elodie, King, Matthew, Mangin, Brigitte, Chaidir, Nadia, Boniface, Marie-Claude, Blanchet, Nicolas, Bret-Mestries, Emmanuelle, Le Paslier, Marie-Christine, Murat, Florent, Helmstetter, Nicolas, Burke, John M., Gill, Navdeep, Bellec, Arnaud, Cottret, Ludovic, Faraut, Thomas, Mayjonade, Baptiste, Thomas, Justine, Staton, S. Evan, Lorenzon, Lolita, Bowers, John E., Bérard, Aurélie, Lai, Zhao, Vincourt, Patrick, Marchand, Gwenaëlle, Nambeesan, Savithri, Carrère, Sébastien

2017

Overview

A high-quality reference for the sunflower genome ( Helianthus annuus L.) and analysis of gene networks involved in flowering time and oil metabolism provide a basis for nutritional exploitation and analyses of adaptation to climate change. Sunflower genome Nicolas Langlade and colleagues report the genome sequence of the domesticated sunflower, Helianthus annuus L., a global oil crop that can maintain stable yields across a wide range of environmental conditions. Their comparative analyses provide insights into the evolutionary history of Asterids. They also analysed transcriptomic data from vegetative and floral organs, re-sequenced 80 domesticated lines and performed genome-wide association studies identifying 35 loci associated with flowering time. These resources will be useful in breeding programs as well as ecological and evolutionary studies. The domesticated sunflower, Helianthus annuus L., is a global oil crop that has promise for climate change adaptation, because it can maintain stable yields across a wide variety of environmental conditions, including drought 1 . Even greater resilience is achievable through the mining of resistance alleles from compatible wild sunflower relatives 2 , 3 , including numerous extremophile species 4 . Here we report a high-quality reference for the sunflower genome (3.6 gigabases), together with extensive transcriptomic data from vegetative and floral organs. The genome mostly consists of highly similar, related sequences 5 and required single-molecule real-time sequencing technologies for successful assembly. Genome analyses enabled the reconstruction of the evolutionary history of the Asterids, further establishing the existence of a whole-genome triplication at the base of the Asterids II clade 6 and a sunflower-specific whole-genome duplication around 29 million years ago 7 . An integrative approach combining quantitative genetics, expression and diversity data permitted development of comprehensive gene networks for two major breeding traits, flowering time and oil metabolism, and revealed new candidate genes in these networks. We found that the genomic architecture of flowering time has been shaped by the most recent whole-genome duplication, which suggests that ancient paralogues can remain in the same regulatory networks for dozens of millions of years. This genome represents a cornerstone for future research programs aiming to exploit genetic diversity to improve biotic and abiotic stress resistance and oil production, while also considering agricultural constraints and human nutritional needs 8 , 9 .

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 45/23 / 45/43 / 631/208/212/2304 / 631/208/726 / 631/449/2669 / 631/449/711 / Acclimatization - genetics / Adaptation / Architecture / Assembly / Biochemistry, Molecular Biology / Botany / Climate / Climate adaptation / Climate change / Compatibility / Crops / Duplicating milling machines / Environmental conditions / Evolution / Evolution, Molecular / Evolutionary genetics / Flowering / Flowers & plants / Flowers - genetics / Flowers - physiology / Gene Duplication - genetics / Gene expression / Gene Expression Regulation, Plant / Gene sequencing / Genes / Genetic aspects / Genetic diversity / Genetic Variation / Genetics / Genome, Plant - genetics / Genomes / Genomics / Helianthus - classification / Helianthus - genetics / Helianthus - metabolism / Helianthus annuus / Humanities and Social Sciences / letter / Life Sciences / Metabolism / Mining / multidisciplinary / Networks / Observations / Oils & fats / Organs / Plant breeding / Plant metabolism / Plant Oils - metabolism / Plants genetics / Quantitative genetics / Real time / Reconstruction / Research programs / Resilience / Science / Sequence Analysis, DNA / Stress, Physiological - genetics / Stresses / Sunflower Oil / Sunflowers / Transcription / Transcriptome - genetics