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Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice
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Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice
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Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice
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Journal Article
Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice
2020
Overview
Embryonic stem cells (ESCs) derived from outbred mice which share several genetic characteristics similar to humans have been requested for developing stem cell-based bioengineering techniques directly applicable to humans. Here, we report the generation of ESCs derived from the inner cell mass of blastocysts retrieved from 9-week-old female outbred ICR mice mated with 9-week-old male outbred ICR mice (
ICR
ESCs). Similar to those from 129/Ola mouse blastocysts (
E14
ESCs), the established
ICR
ESCs showed inherent characteristics of ESCs except for partial and weak protein expression and activity of alkaline phosphatase. Moreover,
ICR
ESCs were not originated from embryonic germ cells or pluripotent cells that may co-exist in outbred ICR strain-derived mouse embryonic fibroblasts (
ICR
MEFs) used for deriving colonies from inner cell mass of outbred ICR mouse blastocysts. Furthermore, instead of outbred
ICR
MEFs, hybrid
B6CBAF1
MEFs as feeder cells could sufficiently support in vitro maintenance of
ICR
ESC self-renewal. Additionally,
ICR
ESC-specific characteristics (self-renewal, pluripotency, and chromosomal normality) were observed in
ICR
ESCs cultured for 40th subpassages (164 days) on
B6CBAF1
MEFs without any alterations. These results confirmed the successful establishment of ESCs derived from outbred ICR mice, and indicated that self-renewal and pluripotency of the established
ICR
ESCs could be maintained on
B6CBAF1
MEFs in culture.
