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An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
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An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
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Journal Article
An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
2024
Overview
Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.
Key points
•aMPV diagnosis using RT-LAMP is achieved with high sensitivity and specificity.
•Fifty field samples have been visualized using DNA-nanoprobe validation.
•The developed system is a reliable, fast, and cost-effective option for POCT.
Graphical Abstract
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Analysis
/ Animals
/ Applied Genetics and Molecular Biotechnology
/ Biomedical and Life Sciences
/ Chickens
/ Cloning
/ DNA
/ Farms
/ Gold
/ Metal Nanoparticles - chemistry
/ Metapneumovirus - isolation & purification
/ Methods
/ Microbial Genetics and Genomics
/ Molecular Diagnostic Techniques - economics
/ Molecular Diagnostic Techniques - methods
/ nanogold
/ Nucleic Acid Amplification Techniques - economics
/ Nucleic Acid Amplification Techniques - methods
/ Paramyxoviridae Infections - diagnosis
/ Paramyxoviridae Infections - veterinary
/ Paramyxoviridae Infections - virology
/ Pharynx
/ Poultry
/ Poultry Diseases - diagnosis
/ Proteins
/ reverse transcription loop-mediated isothermal amplification
/ Turkeys
/ Vaccines
/ Viruses
