Asset Details
Do you wish to reserve the book?
circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer
Do you wish to request the book?
circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer
2019
Overview
Background
Increasing studies has found that circular RNAs (circRNAs) play vital roles in cancer progression. But the expression profile and function of circRNAs in triple-negative breast cancer (TNBC) are unclear.
Methods
We used a circRNA microarray to explore the circRNA expression profile of TNBC. The expression of the top upregulated circRNA, circKIF4A, was confirmed by qRT-PCR in breast cancer cell lines and tissues. Kaplan-Meier survival analysis was conducted to analyze the clinical impact of circKIF4A on TNBC. A series of experiments was performed to explore the functions of circKIF4A in TNBC progression, such as cell proliferation and migration. We investigated the regulatory effect of circKIF4A on miRNA and its target genes to explore the potential regulatory mechanisms of circKIF4A in TNBC.
Results
qRT-PCR analyses verified that circKIF4A was significantly upregulated and positively associated with poorer survival of TNBC. The inhibition of circKIF4A suppressed cell proliferation and migration in TNBC. Luciferase reporter assay and RNA immunoprecipitation assay revealed that circKIF4A and KIF4A could bind to miR-375 and that circKIF4A regulated the expression of KIF4A via sponging miR-375.
Conclusions
The circKIF4A-miR-375-KIF4A axis regulates TNBC progression via the competitive endogenous RNA (ceRNA) mechanism. circKIF4A may therefore serve as a prognostic biomarker and therapeutic target for TNBC.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Aged
/ Animals
/ Biomedical and Life Sciences
/ Female
/ Gene Expression Regulation, Neoplastic
/ Genes
/ Humans
/ KIF4A
/ Mice
/ MicroRNA
/ miR-375
/ miRNA
/ Oncology
/ Proteins
/ RNA
/ RNA - antagonists & inhibitors
/ RNA, Small Interfering - genetics
/ RNA, Small Interfering - metabolism
/ Triple negative breast cancer
/ Triple Negative Breast Neoplasms - diagnosis
/ Triple Negative Breast Neoplasms - genetics
/ Triple Negative Breast Neoplasms - mortality
