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Anti-H7N9 avian influenza A virus activity of interferon in pseudostratified human airway epithelium cell cultures
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Anti-H7N9 avian influenza A virus activity of interferon in pseudostratified human airway epithelium cell cultures
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Journal Article
Anti-H7N9 avian influenza A virus activity of interferon in pseudostratified human airway epithelium cell cultures
2019
Overview
Background
Since H7N9 influenza A virus (H7N9) was first reported in 2013, five waves of outbreaks have occurred, posing a huge threat to human health. In preparation for a potential H7N9 epidemic, it is essential to evaluate the efficacy of anti-H7N9 drugs with an appropriate model.
Methods
Well-differentiated pseudostratified human airway epithelium (HAE) cells were grown at the air–liquid interface, and the H7N9 cell tropism and cytopathic effect were detected by immunostaining and hematoxylin-eosin (HE) staining. The H7N9 replication kinetics and anti-H7N9 effect of recombinant human α2b (rhIFN-α2b) and rhIFN-λ1 were compared with different cell lines. The H7N9 viral load and interferon-stimulated gene (ISG) expression were quantified by real-time PCR assays.
Results
H7N9 could infect both ciliated and non-ciliated cells within the three-dimensional (3D) HAE cell culture, which reduced the number of cilia and damaged the airways. The H7N9 replication kinetics differed between traditional cells and 3D HAE cells. Interferon had antiviral activity against H7N9 and alleviated epithelial cell lesions; the antiviral activity of rhIFN-α2b was slightly better than that of rhIFN-λ1. In normal cells, rhIFN-α2b induced a greater amount of ISG expression (MX1, OAS1, IFITM3, and ISG15) compared with rhIFN-λ1, but in 3D HAE cells, this trend was reversed.
Conclusions
Both rhIFN-α2b and rhIFN-λ1 had antiviral activity against H7N9, and this protection was related to the induction of ISGs. The 3D cell culture model is suitable for evaluating interferon antiviral activity because it can demonstrate realistic in vivo-like effects.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Antiviral Agents - pharmacology
/ Biological response modifiers
/ Biomedical and Life Sciences
/ Cilia
/ Control
/ drugs
/ Genes
/ H7N9
/ Health
/ Humans
/ Influenza A Virus, H7N9 Subtype - drug effects
/ Influenza A Virus, H7N9 Subtype - immunology
/ Interferon alpha-2 - pharmacology
/ Membrane Proteins - genetics
/ Myxovirus resistance proteins
/ Myxovirus Resistance Proteins - genetics
/ Proteins
/ quantitative polymerase chain reaction
/ RNA-Binding Proteins - genetics
/ staining
/ Tropism
/ Vaccines
/ Virology
/ Virus Replication - drug effects
/ Viruses
