Journal Article

Metabolomic signatures of the long-term exposure to air pollution and temperature

Nassan, Feiby L., Lasky-Su, Jessica A., Vokonas, Pantel S., Kelly, Rachel S., Koutrakis, Petros, Kosheleva, Anna, Schwartz, Joel D.

2021

Overview

Background Long-term exposures to air pollution has been reported to be associated with inflammation and oxidative stress. However, the underlying metabolic mechanisms remain poorly understood. Objectives We aimed to determine the changes in the blood metabolome and thus the metabolic pathways associated with long-term exposure to outdoor air pollution and ambient temperature. Methods We quantified metabolites using mass-spectrometry based global untargeted metabolomic profiling of plasma samples among men from the Normative Aging Study (NAS). We estimated the association between long-term exposure to PM 2.5 , NO 2 , O 3 , and temperature (annual average of central site monitors) with metabolites and their associated metabolic pathways. We used multivariable linear mixed-effect regression models (LMEM) while simultaneously adjusting for the four exposures and potential confounding and correcting for multiple testing. As a reduction method for the intercorrelated metabolites (outcome), we further used an independent component analysis (ICA) and conducted LMEM with the same exposures. Results Men ( N  = 456) provided 648 blood samples between 2000 and 2016 in which 1158 metabolites were quantified. On average, men were 75.0 years and had an average body mass index of 27.7 kg/m 2 . Almost all men (97%) were not current smokers. The adjusted analysis showed statistically significant associations with several metabolites (58 metabolites with PM 2.5 , 15 metabolites with NO 2 , and 6 metabolites with temperature) while no metabolites were associated with O 3 . One out of five ICA factors (factor 2) was significantly associated with PM 2.5 . We identified eight perturbed metabolic pathways with long-term exposure to PM 2.5 and temperature: glycerophospholipid, sphingolipid, glutathione, beta-alanine, propanoate, and purine metabolism, biosynthesis of unsaturated fatty acids, and taurine and hypotaurine metabolism. These pathways are related to inflammation, oxidative stress, immunity, and nucleic acid damage and repair. Conclusions Using a global untargeted metabolomic approach, we identified several significant metabolites and metabolic pathways associated with long-term exposure to PM 2.5 , NO 2 and temperature. This study is the largest metabolomics study of long-term air pollution, to date, the first study to report a metabolomic signature of long-term temperature exposure, and the first to use ICA in the analysis of both.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Aging / Air pollution / Air temperature / Alanine / Ambient temperature / Biosynthesis / Blood / Body mass index / Body size / Earth and Environmental Science / Environment / Environmental Epidemiology / Environmental Health / Exposure / Fatty acids / Glutathione / Health aspects / Humidity / Independent component analysis / Inflammation / Medical research / Medicine, Experimental / Metabolic pathways / Metabolism / Metabolites / Metabolomics / Mortality / Nitrogen dioxide / Normative aging study (NAS) / Nucleic acids / Occupational Medicine/Industrial Medicine / Outdoor air quality / Oxidative stress / Particles / Particulate matter / Physiological aspects / Pollutants / Public Health / Regression analysis / Regression models / Signatures / Spectrometry / Statistical analysis / Statistical methods / Taurine / Temperature