Journal Article

Elevated peripheral inflammation is associated with choroid plexus enlargement in independent sporadic amyotrophic lateral sclerosis cohorts

Yun, Yan, Chen, Yujing, Yan, Chuanzhu, Sun, Sujuan, Liu, Shuangwu, Sun, Xiaohan, Meng, Xiangshui, Lin, Pengfei, Zhao, Bing, Ren, Qingguo

2024

Overview

Background Using neuroimaging techniques, growing evidence has suggested that the choroid plexus (CP) volume is enlarged in multiple neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). Notably, the CP has been suggested to play an important role in inflammation-induced CNS damage under disease conditions. However, to our knowledge, no study has investigated the relationships between peripheral inflammation and CP volume in sporadic ALS patients. Thus, in this study, we aimed to verify CP enlargement and explore its association with peripheral inflammation in vivo in independent ALS cohorts. Methods Based on structural MRI data, CP volume was measured using Gaussian mixture models and further manually corrected in two independent cohorts of sporadic ALS patients and healthy controls (HCs). Serum inflammatory protein levels were measured using a novel high-sensitivity Olink proximity extension assay (PEA) technique. Xtreme gradient boosting (XGBoost) was used to explore the contribution of peripheral inflammatory factors to CP enlargement. Then, partial correlation analyses were performed. Results CP volumes were significantly higher in ALS patients than in HCs in the independent cohorts. Compared with HCs, serum levels of CRP, IL-6, CXCL10, and 35 other inflammatory factors were significantly increased in ALS patients. Using the XGBoost approach, we established a model-based importance of features, and the top three predictors of CP volume in ALS patients were CRP, IL-6, and CXCL10 (with gains of 0.24, 0.18, and 0.15, respectively). Correlation analyses revealed that CRP, IL-6, and CXCL10 were significantly associated with CP volume in ALS patients ( r  = 0.462 ∼ 0.636, p <  0.001). Conclusion Our study is the first to reveal a consistent and replicable contribution of peripheral inflammation to CP enlargement in vivo in sporadic ALS patients. Given that CP enlargement has been recently detected in other brain diseases, these findings should consider extending to other disease conditions with a peripheral inflammatory component.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adult / Aged / Algorithms / ALS / Amyotrophic lateral sclerosis / Amyotrophic Lateral Sclerosis - blood / Amyotrophic Lateral Sclerosis - diagnostic imaging / Amyotrophic Lateral Sclerosis - pathology / Biomarkers / Biomedical and Life Sciences / Biomedicine / Brain diseases / Chitinase / Choroid plexus / Choroid Plexus - diagnostic imaging / Choroid Plexus - pathology / Cohort Studies / Correlation analysis / CXCL10 protein / Enlargement / Family medical history / Female / Genetic testing / Hematology / Hospitals / Humans / Inflammation / Inflammation - blood / Inflammation - diagnostic imaging / Interleukin 6 / Machine learning / Magnetic Resonance Imaging / Male / Medical colleges / Medical research / Medicine, Experimental / Middle Aged / MRI / Mutation / Nervous system diseases / Neurobiology / Neurodegenerative diseases / Neuroimaging / Neurosciences / Proteins / Psychosis / Serum levels / Somatotropin