Journal Article

LncRNA MT1JP functions as a ceRNA in regulating FBXW7 through competitively binding to miR-92a-3p in gastric cancer

Li, Shuwei, Du, Mulong, Wang, Qiaoyan, Wang, Meilin, Chu, Haiyan, Lu, Jiafei, Gong, Weida, Zhang, Aihua, Zhang, Zhengdong, Zhang, Gang, Ge, Yuqiu, Ma, Gaoxiang, Zhao, Qinghong, Wu, Dongdong

2018

Overview

Background Emerging evidence has shown that dysregulation function of long non-coding RNAs (lncRNAs) implicated in gastric cancer (GC). However, the role of the differentially expressed lncRNAs in GC has not fully explained. Methods LncRNA expression profiles were determined by lncRNA microarray in five pairs of normal and GC tissues, further validated in another 75 paired tissues by quantitative real-time PCR (qRT-PCR). Overexpression of lncRNA MT1JP was conducted to assess the effect of MT1JP in vitro and in vivo. The biological functions were demonstrated by luciferase reporter assay, western blotting and rescue experiments. Results LncRNA MT1JP was significantly lower in GC tissues than adjacent normal tissues, and higher MT1JP was remarkably related to lymph node metastasis and advance stage. Besides, GC patients with higher MT1JP expression had a well survival. Functionally, overexpression of lncRNA MT1JP inhibited cell proliferation, migration, invasion and promoted cell apoptosis in vitro, and inhibited tumor growth and metastasis in vivo. Functional analysis showed that lncRNA MT1JP regulated FBXW7 expression by competitively binding to miR-92a-3p. MiR-92a-3p and down-regulated FBXW7 reversed cell phenotypes caused by lncRNA MT1JP by rescue analysis. Conclusion MT1JP , a down-regulated lncRNA in GC, was associated with malignant tumor phenotypes and survival of GC. MT1JP regulated the progression of GC by functioning as a competing endogenous RNA (ceRNA) to competitively bind to miR-92a-3p and regulate FBXW7 expression. Our study provided new insight into the post-transcriptional regulation mechanism of lncRNA MT1JP , and suggested that MT1JP may act as a potential therapeutic target and prognosis biomarker for GC.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Animals / Biological markers / Biomedical and Life Sciences / Biomedicine / Cancer Research / Care and treatment / Cell Movement / Cell Proliferation / ceRNA / Development and progression / Down-Regulation / F-Box-WD Repeat-Containing Protein 7 - genetics / Female / Gastric cancer / Gene expression / Gene Expression Regulation, Neoplastic / Humans / lncRNA / Lymphatic Metastasis / Mice / MicroRNAs - genetics / MT1JP / Neoplasm Staging / Neoplasm Transplantation / Oligonucleotide Array Sequence Analysis / Oncology / Prevention / Prognosis / Real-Time Polymerase Chain Reaction / RNA, Long Noncoding - genetics / Stomach cancer / Stomach Neoplasms - genetics / Stomach Neoplasms - pathology