Journal Article

The bionomics of the malaria vector Anopheles rufipes Gough, 1910 and its susceptibility to deltamethrin insecticide in North Cameroon

Toto, Jean Claude, Donnelly, Martin, Ndo, Cyrille, Mnzava, Abraham P., Eyisap, Wolfgang Ekoko, Fondjo, Etienne, Piameu, Michael C., Mandeng, Elysée S., Patchoke, Salomon, Etang, Josiane, Knox, Tessa B., D. Bigoga, Jude, Antonio-Nkondjio, Christophe, Awono-Ambene, Parfait H., Mbakop, Ranaise L.

2018

Overview

Background Following the recent discovery of the role of Anopheles rufipes Gough, 1910 in human malaria transmission in the northern savannah of Cameroon, we report here additional information on its feeding and resting habits and its susceptibility to the pyrethroid insecticide deltamethrin. Methods From 2011 to 2015, mosquito samples were collected in 38 locations across Garoua, Mayo Oulo and Pitoa health districts in North Cameroon. Adult anophelines collected using outdoor clay pots, window exit traps and indoor spray catches were checked for feeding status, blood meal origin and Plasmodium circumsporozoite protein. The susceptibility of field-collected An. rufipes to deltamethrin was assessed using WHO standard procedures. Results Of 9327 adult Anopheles collected in the 38 study sites, An. rufipes (6.5%) was overall the fifth most abundant malaria vector species following An. arabiensis (52.4%), An. funestus ( s.l. ) (20.8%), An. coluzzii (12.6%) and An. gambiae (6.8%). This species was found outdoors (51.2%) or entering houses (48.8%) in 35 suburban and rural locations, together with main vector species. Apart from human blood with index of 37%, An. rufipes also fed on animals including cows (52%), sheep (49%), pigs (16%), chickens (2%) and horses (1%). The overall parasite infection rate of this species was 0.4% based on the detection of P. falciparum circumsporozoite proteins in two of 517 specimens tested. Among the 21 An. rufipes populations assessed for deltamethrin susceptibility, seven populations were classified as “susceptible” (mortality ≥ 98%) , ten as “probable resistant” with a mortality range of 90–97% and four as “resistant” with a mortality range of 80–89%. Conclusions This study revealed changeable resting and feeding behaviour of An. rufipes , as well as further evidence on its ability to carry human malaria parasites in North Cameroon. Besides, this species is developing physiological resistance to deltamethrin insecticide which is used in treated nets and agriculture throughout the country, and should be regarded as one of potential targets for the control of residual malaria parasite transmission in Africa.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: adults / Animals / Anopheles / Anopheles - drug effects / Anopheles - physiology / Anopheles rufipes / Behavior, Animal / Biomedical and Life Sciences / Biomedicine / Bionomics / blood / blood meal / Cameroon / Cameroon - epidemiology / Cattle / clay / Control / deltamethrin / Deltamethrin susceptibility / Disease Vectors / Ecological and Environmental Phenomena / Entomology / Female / Humans / Infectious Diseases / Insecticide Resistance / Insecticides - pharmacology / Malaria / Malaria - epidemiology / Malaria - parasitology / Malaria - prevention & control / Malaria - transmission / Malaria vector / Malaria, Falciparum - parasitology / mortality / Mosquito Control - methods / mosquito vectors / Mosquito Vectors - drug effects / Nitriles - pharmacology / parasites / Parasitology / Plasmodium falciparum / Plasmodium falciparum - drug effects / Prevention / pyrethrins / Pyrethrins - pharmacology / savannas / sheep / species / Testing / Tropical Medicine / Veterinary Medicine/Veterinary Science / Virology