Journal Article

Effect of bacillus subtilis strain Z15 secondary metabolites on immune function in mice

Zhang, Hui, Zhang, Qi, Chen, Jia-Yi, Yang, Jun, Zhao, Jing-Jing, Zhao, He-Ping, Chen, Zhong-Yi, You, Jia, Mao, Qing-Chen, Yang, Li-Ping, Li, Hao-Ran, Yin, Li, Zhao, Hui-Xin, Yu, Fei, Yang, Jing, Cao, Xi-Yuan, Aimaier, Reyihanguli

2023

Overview

Background Previous studies have shown that secondary metabolites of Bacillus subtilis strain Z15 (BS-Z15) are effective in treating fungal infections in mice. To evaluate whether it also modulates immune function in mice to exert antifungal effects, we investigated the effect of BS-Z15 secondary metabolites on both the innate and adaptive immune functions of mice, and explored its molecular mechanism through blood transcriptome analysis. Results The study showed that BS-Z15 secondary metabolites increased the number of monocytes and platelets in the blood, improved natural killer (NK) cell activity and phagocytosis of monocytes-macrophages, increased the conversion rate of lymphocytes in the spleen, the number of T lymphocytes and the antibody production capacity of mice, and increased the levels of Interferon gamma (IFN-γ), Interleukin-6 (IL-6), Immunoglobulin G (IgG) and Immunoglobulin M (IgM) in plasma. The blood transcriptome analysis revealed 608 differentially expressed genes following treatment with BS-Z15 secondary metabolites, all of which were significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms for immune-related entries and pathways such as Tumor Necrosis Factor (TNF) and Toll-like receptor (TLR) signaling pathways, and upregulated expression levels of immune-related genes such as Complement 1q B chain (C1qb), Complement 4B (C4b), Tetracyclin Resistant (TCR) and Regulatory Factor X, 5 (RFX5). Conclusions BS-Z15 secondary metabolites were shown to enhance innate and adaptive immune function in mice, laying a theoretical foundation for its development and application in the field of immunity.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abdomen / Analysis / Animal Genetics and Genomics / Animals / Antifungal agents / Bacillus subtilis / Biological response modifiers / Biomedical and Life Sciences / Blood / Care and treatment / Chromosome 1 / Coagulation factors / Disease / Drugs / Encyclopedias / Fungicides / Gene expression / Genes / Genomes / Genomics / Health aspects / IgG antibody / Immune function / Immune response / Immune system / Immunoglobulin G / Immunoglobulin M / Immunoglobulins / Infection / Interferon / Interferon-gamma / Interleukin 6 / Interleukins / Killer Cells, Natural - metabolism / Life Sciences / Lymphocytes / Lymphocytes T / Macrophages / Metabolites / Mice / Microarrays / Microbial Genetics and Genomics / Molecular modelling / Monocytes / Mycoses / Natural killer cells / Phagocytosis / Physiological aspects / Physiology / Plant Genetics and Genomics / Plant metabolites / Probiotics / Proteins / Proteomics / Resistance factors / Secondary metabolites / Spleen / T cell receptors / T cells / T-Lymphocytes - metabolism / Testing / Toll-like receptors / Transcriptome / Transcriptomes / Veins & arteries / γ-Interferon