Journal Article

Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects

Mori, Yasuyoshi, Tomita, Norihiro, Notomi, Tsugunori, Kanda, Hidetoshi

2015

Overview

Loop-mediated isothermal amplification (LAMP), a newly developed gene amplification method, combines rapidity, simplicity, and high specificity. Several tests have been developed based on this method, and simplicity is maintained throughout all steps, from extraction of nucleic acids to detection of amplification. In the LAMP reaction, samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions: self-elongation of templates from the stem loop structure formed at the 3′-terminal and the binding and elongation of new primers to the loop region. The LAMP reaction has a wide range of possible applications, including point-of-care testing, genetic testing in resource-poor settings (such as in developing countries), and rapid testing of food products and environmental samples.

• Publisher: Springer-Verlag,The Microbiological Society of Korea,Springer Nature B.V,한국미생물학회
• Subject: Biomedical and Life Sciences / Design / Developing countries / DNA Primers / Enzymes / Food products / foods / Gene amplification / Genetic disorders / Genetic screening / Genetic Testing / Humans / Influenza / Inverted Repeat Sequences / LDCs / Life Sciences / loop-mediated isothermal amplification / Methods / Microbiology / Minireview / Nucleic Acid Amplification Techniques / Nucleic acids / Orthomyxoviridae - isolation & purification / Point of care testing / Point-of-Care Systems / Polymerase chain reaction / R&D / Reagents / Research & development / Software / Temperature / Tuberculosis - diagnosis / 생물학