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Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

by Klassen, Levi , Sloan, Angela , Salawudeen, Abd’jeleel , Tailor, Nikesh , Soule, Geoff , Deschambault, Yvon , Safronetz, David , Audet, Jonathan

in Animal experimentation / Animal models / Animals / Antibodies, Viral - blood / Antibodies, Viral - immunology / Developing countries / Disease Models, Animal / Dispatch / Drug stability / Ebola virus / Freeze Drying / Glycoproteins / Guinea Pigs / in Animal Model / Infectious diseases / Lassa fever / Lassa virus / LDCs / lyophilized / Marburg virus disease / Methods / Pathogens / Pharmaceutical research / Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines / Public health / RNA viruses / Stomatitis / Temperature / Testing / Vaccine Efficacy / Vaccines / Vesicular Stomatitis - immunology / Vesicular Stomatitis - prevention & control / Vesicular Stomatitis - virology / Vesicular stomatitis Indiana virus - immunology / vesicular stomatitis virus / Vesiculovirus - genetics / Vesiculovirus - immunology / Viral proteins / Viral vaccines / Viral Vaccines - administration & dosage / Viral Vaccines - immunology / Viruses

2024

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Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

by Klassen, Levi , Sloan, Angela , Salawudeen, Abd’jeleel , Tailor, Nikesh , Soule, Geoff , Deschambault, Yvon , Safronetz, David , Audet, Jonathan

2024

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Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

by Klassen, Levi , Sloan, Angela , Salawudeen, Abd’jeleel , Tailor, Nikesh , Soule, Geoff , Deschambault, Yvon , Safronetz, David , Audet, Jonathan

2024

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Journal Article

Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

Klassen, Levi,

Sloan, Angela,

Salawudeen, Abd’jeleel,

Tailor, Nikesh,

Soule, Geoff,

Deschambault, Yvon,

Safronetz, David,

Audet, Jonathan

2024

Overview

We evaluated the in vitro effects of lyophilization for 2 vesicular stomatitis virus-based vaccines by using 3 stabilizing formulations and demonstrated protective immunity of lyophilized/reconstituted vaccine in guinea pigs. Lyophilization increased stability of the vaccines, but specific vesicular stomatitis virus-based vaccines will each require extensive analysis to optimize stabilizing formulations.

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Publisher

U.S. National Center for Infectious Diseases,Centers for Disease Control and Prevention

Subject

Animal experimentation

/ Animal models

/ Animals

/ Antibodies, Viral - blood

/ Antibodies, Viral - immunology

/ Developing countries

/ Disease Models, Animal