Journal Article

An easier life to come for mosquito researchers: field-testing across Italy supports VECTRACK system for automatic counting, identification and absolute density estimation of Aedes albopictus and Culex pipiens adults

Di Lillo, Paola, Epis, Sara, Salvemini, Marco, Montarsi, Fabrizio, Bernardini, Ilaria, Micocci, Martina, Poletti, Piero, Manica, Mattia, Severini, Francesco, Varone, Marianna, Soresinetti, Laura, Caputo, Beniamino, della Torre, Alessandra

2024

Overview

Background Disease-vector mosquito monitoring is an essential prerequisite to optimize control interventions and evidence-based risk predictions. However, conventional entomological monitoring methods are labor- and time-consuming and do not allow high temporal/spatial resolution. In 2022, a novel system coupling an optical sensor with machine learning technologies (VECTRACK) proved effective in counting and identifying Aedes albopictus and Culex pipiens adult females and males. Here, we carried out the first extensive field evaluation of the VECTRACK system to assess: (i) whether the catching capacity of a commercial BG-Mosquitaire trap (BGM) for adult mosquito equipped with VECTRACK (BGM + VECT) was affected by the sensor; (ii) the accuracy of the VECTRACK algorithm in correctly classifying the target mosquito species genus and sex; (iii) Ae. albopictus capture rate of BGM with or without VECTRACK. Methods The same experimental design was implemented in four areas in northern (Bergamo and Padua districts), central (Rome) and southern (Procida Island, Naples) Italy. In each area, three types of traps—one BGM, one BGM + VECT and the combination of four sticky traps (STs)—were rotated each 48 h in three different sites. Each sampling scheme was replicated three times/area. Collected mosquitoes were counted and identified by both the VECTRACK algorithm and operator-mediated morphological examination. The performance of the VECTRACK system was assessed by generalized linear mixed and linear regression models. Aedes albopictus capture rates of BGMs were calculated based on the known capture rate of ST. Results A total of 3829 mosquitoes (90.2% Ae. albopictus ) were captured in 18 collection-days/trap/site. BGM and BGM + VECT showed a similar performance in collecting target mosquitoes. Results show high correlation between visual and automatic identification methods (Spearman Ae. albopictus : females = 0.97; males = 0.89; P  < 0.0001) and low count errors. Moreover, the results allowed quantifying the heterogeneous effectiveness associated with different trap types in collecting Ae. albopictus and predicting estimates of its absolute density. Conclusions Obtained results strongly support the VECTRACK system as a powerful tool for mosquito monitoring and research, and its applicability over a range of ecological conditions, accounting for its high potential for continuous monitoring with minimal human effort. Graphical Abstract

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Aedes - classification / Aedes - physiology / Aedes albopictus / Algorithms / Analysis / Animals / Automatic identification / Biomedical and Life Sciences / Biomedicine / Control / Culex - classification / Culex - physiology / Culex pipiens / Entomology / Environmental monitoring / Female / genus / humans / Identification and classification / imagos / Infectious Diseases / Italy / Machine Learning / Male / Mosquito Control - instrumentation / Mosquito Control - methods / Mosquito trap / Mosquito Vectors - classification / Mosquito Vectors - physiology / Mosquitoes / Optical detectors / Optical sensor / Parasitology / Population Density / regression analysis / risk / Sensors / species / Technology application / Tropical Medicine / Veterinary Medicine/Veterinary Science / Virology