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A portable transistor immunosensor for fast identification of porcine epidemic diarrhea virus
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A portable transistor immunosensor for fast identification of porcine epidemic diarrhea virus
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Journal Article
A portable transistor immunosensor for fast identification of porcine epidemic diarrhea virus
2024
Overview
Widespread distribution of porcine epidemic diarrhea virus (PEDV) has led to catastrophic losses to the global pig farming industry. As a result, there is an urgent need for rapid, sensitive and accurate tests for PEDV to enable timely and effective interventions. In the present study, we develop and validate a floating gate carbon nanotubes field-effect transistor (FG CNT-FET)-based portable immunosensor for rapid identification of PEDV in a sensitive and accurate manner. To improve the affinity, a unique PEDV spike protein-specific monoclonal antibody is prepared by purification, and subsequently modified on FG CNT-FET sensor to recognize PEDV. The developed FET biosensor enables highly sensitive detection (LoD: 8.1 fg/mL and 10
0.14
TCID
50
/mL for recombinant spike proteins and PEDV, respectively), as well as satisfactory specificity. Notably, an integrated portable platform consisting of a pluggable FG CNT-FET chip and a portable device can discriminate PEDV positive from negative samples and even identify PEDV and porcine deltacoronavirus within 1 min with 100% accuracy. The portable sensing platform offers the capability to quickly, sensitively and accurately identify PEDV, which further points to a possibility of point of care (POC) applications of large-scale surveillance in pig breeding facilities.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Antibodies, Monoclonal - immunology
/ Antibodies, Viral - immunology
/ Biosensing Techniques - instrumentation
/ Biosensing Techniques - methods
/ Chemistry and Materials Science
/ Coronavirus Infections - diagnosis
/ Coronavirus Infections - veterinary
/ Coronavirus Infections - virology
/ Diseases
/ Identification and classification
/ Immunoassay - instrumentation
/ Nanotubes, Carbon - chemistry
/ Porcine epidemic diarrhea virus
/ Porcine epidemic diarrhea virus - isolation & purification
/ Spike Glycoprotein, Coronavirus - analysis
/ Spike Glycoprotein, Coronavirus - immunology
/ Swine
/ Testing
