Journal Article

Prediction of hot spots in protein–DNA binding interfaces based on supervised isometric feature mapping and extreme gradient boosting

Bin, Yannan, Xia, Junfeng, Yan, Di, Zhang, Sijia, Li, Ke

2020

Overview

Background Identification of hot spots in protein-DNA interfaces provides crucial information for the research on protein-DNA interaction and drug design. As experimental methods for determining hot spots are time-consuming, labor-intensive and expensive, there is a need for developing reliable computational method to predict hot spots on a large scale. Results Here, we proposed a new method named sxPDH based on supervised isometric feature mapping (S-ISOMAP) and extreme gradient boosting (XGBoost) to predict hot spots in protein-DNA complexes. We obtained 114 features from a combination of the protein sequence, structure, network and solvent accessible information, and systematically assessed various feature selection methods and feature dimensionality reduction methods based on manifold learning. The results show that the S-ISOMAP method is superior to other feature selection or manifold learning methods. XGBoost was then used to develop hot spots prediction model sxPDH based on the three dimensionality-reduced features obtained from S-ISOMAP. Conclusion Our method sxPDH boosts prediction performance using S-ISOMAP and XGBoost. The AUC of the model is 0.773, and the F1 score is 0.713. Experimental results on benchmark dataset indicate that sxPDH can achieve generally better performance in predicting hot spots compared to the state-of-the-art methods.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Accuracy / Algorithms / Amino acid sequence / Analysis / Binding sites / Bioinformatics / Biomedical and Life Sciences / Computational Biology/Bioinformatics / Computer Appl. in Life Sciences / Computer applications / Datasets / Deoxyribonucleic acid / DNA / DNA binding / Drug development / Experimental methods / Extreme gradient boosting / Feature selection / Genetic research / Hot spot / Interfaces / Isometric / Life Sciences / Machine learning / Manifolds (mathematics) / Methodology / Microarrays / Neighborhoods / Peptide mapping / Performance prediction / Prediction models / Protein binding / Protein structure / Proteins / Protein–DNA complexes / Research methodology / Supervised isometric feature mapping