Journal Article

Nucleocapsid single point-mutation associated with drop-out on RT-PCR assay for SARS-CoV-2 detection

Pinho, João Renato Rebello, Marra, Alexandre Rodrigues, da Silva Nali, Luiz Henrique, Petroni, Roberta Cardoso, de Mello Malta, Fernanda, Neto, Miguel Cendoroglo, Oler, Silvia Cassiano, Siqueira, Ricardo Andreotti, Amgarten, Deyvid

2023

Overview

Background Since its beginning, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been a challenge for clinical and molecular diagnostics, because it has been caused by a novel viral agent. Whole-genome sequencing assisted in the characterization and classification of SARS-CoV-2, and it is an essential tool to genomic surveillance aiming to identify potentials hot spots that could impact on vaccine immune response and on virus diagnosis. We describe two cases of failure at the N2 target of the RT-PCR test Xpert® Xpress SARS-CoV-2. Methods Total nucleic acid from the Nasopharyngeal (NP) and oropharyngeal (OP) swab samples and cell supernatant isolates were obtained. RNA samples were submitted to random amplification. Raw sequencing data were subjected to sequence quality controls, removal of human contaminants by aligning against the HG19 reference genome, taxonomic identification of other pathogens and genome recovery through assembly and manual curation. RT-PCR test Xpert® Xpress SARS-CoV-2 was used for molecular diagnosis of SARS-CoV-2 infection, samples were tested in duplicates. Results We identified 27 samples positive for SARS-CoV-2 with a nucleocapsid (N) gene drop out on Cepheid Xpert® Xpress SARS-CoV-2 assay. Sequencing of 2 of 27 samples revealed a single common mutation in the N gene C29197T, potentially involved in the failed detection of N target. Conclusions This study highlights the importance of genomic data to update molecular tests and vaccines.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Acids / Analysis / Brazil / Contaminants / Coronaviruses / COVID-19 / COVID-19 vaccines / Diagnosis / DNA sequencing / Einstein, Albert (1879-1955) / Epidemics / Gene sequencing / Genes / Genetic aspects / Genomes / Genomic data / Genomics / Health aspects / Immune response / Immunization / Infections / Infectious Diseases / Internal Medicine / Medical Microbiology / Medicine / Medicine & Public Health / Microbial mutation / Molecular tests / Mutation / N gene / Nucleic acids / Nucleocapsids / Nucleotide sequencing / Pandemics / Parasitology / Polymerase chain reaction / Proteins / Quality control / RNA / SARS-CoV-2 / Severe acute respiratory syndrome coronavirus 2 / Software / Target detection / Tropical Medicine / Vaccines / Viral agent / Viral diseases / Whole genome sequencing