Journal Article

The effects of saline water consumption on sperm parameters, testicular histopathology, hormonal and antioxidants concentrations in Barki Rams

Hussein, Hassan A., Mohamed, Ragab H, Tahoun, Enas A., Mohamed, Rasha S., Wehrend, Axel

2024

Overview

The study aimed to assess the effects of water salinity on the sperm parameters, levels of cortisol, LH, FSH, testosterone and antioxidants as well as the testes’ histopathology in Barki rams. Fifteen healthy Barki rams (1–1.5 years) were divided into three equal depending on the type of drinking water for nine months. The rams in the tap water group (TW, water that contained 350 ppm of total dissolved salts (TDS). Males in the high saline water group (HSW) were permitted to consume high saline water with 8,934 ppm TDS, whereas those in the second group were permitted to have moderately saline water (MSW, 4,557 ppm TDS). High salt concentration in drinking water had adverse effect on sperm viability, morphology and sperm cell concertation. Nitric oxide and malondialdehyde concentrations in blood were significantly higher in the MSW and HSW groups than in TW. There was a significant decrease in glutathione concentration as well as superoxide dismutase activity in TDS and HSW. Cortisol was most highly concentrated in the HSW, next in the MSW, and least in TW. The testosterone, LH, and FSH concentrations in the HSW and MSW groups were significantly lower than in TW. As the salt concentration in drinking water increases, damage to testicular tissue. The MSW group demonstrating vacuolation of lining epithelial cells with pyknotic nuclei in the epididymis and necrosis and desquamation of spermatogenic cells in seminiferous tubules while HSW group displaying desquamated necrotic cells and giant cell formation in the epididymis, as well as damage to some of the seminiferous tubules and showed congestion, vacuolation of spermatogenic epithelium of seminiferous tubules, and desquamated necrotic spermatogenic epithelium. In conclusion, the salinity of the water has detrimental impacts on the sperm morphology, viability and concentration, hormones and antioxidant levels in Barki rams.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: adverse effects / Animals / Antioxidants / Antioxidants - metabolism / blood / Climatic change / Corticosteroids / Cortisol / Cytology / Deserts / Drinking water / Dryness / Epididymis / Epithelial cells / Epithelium / Experiments / Follicle Stimulating Hormone - blood / Follicle-stimulating hormone / Food / giant cells / Glutathione / Health aspects / Histochemistry / histopathology / Hydrocortisone - blood / Livestock / Luteinizing hormone / Luteinizing Hormone - blood / Male / malondialdehyde / Measurement / Medicine / Medicine & Public Health / Morphology / Motility / necrosis / Nitric oxide / Oxidative stress / Physiological aspects / Ram / Rams / Reproduction / Rural areas / Saline water / Saline Waters / Salinity / Salinity effects / salt concentration / Sheep / Sperm / Spermatozoa / Spermatozoa - drug effects / Steroid hormones / Superoxide / superoxide dismutase / tap water / Testes / Testis - drug effects / Testis - pathology / Testosterone / Testosterone - blood / Transgenics / Tubules / Veterinary Medicine/Veterinary Science / viability / Water salinity / Zoology