Journal Article

Carbohydrate intake attenuates post-exercise plasma levels of cytochrome P450-generated oxylipins

Nieman, David C., Stephan, Ella H., Zhang, Qibin, Sakaguchi, Camila A., Gillitt, Nicholas D., Chen, Guan-Yuan

2019

Overview

Oxylipins are bioactive oxidation products derived from n-6 and n-3 polyunsaturated fatty acids (PUFAs) in the linoleic acid and α-linolenic desaturation pathways. This study determined if carbohydrate intake during prolonged and intensive cycling countered post-exercise increases in n-6 and n-3 PUFA-derived oxylipins. The research design utilized a randomized, crossover, counterbalanced approach with cyclists (N = 20, overnight fasted state, 7:00 am start) who engaged in four 75-km time trials while ingesting two types of bananas (Cavendish, Mini-yellow), a 6% sugar beverage, and water only. Carbohydrate intake was set at 0.2 g/kg every 15 minutes, and blood samples were collected pre-exercise and 0 h-, 0.75 h-,1.5 h-, 3 h-, 4.5 h-, 21 h-, 45 h-post-exercise. Oxylipins were measured with a targeted liquid chromatography-multiple reaction monitoring mass spectrometric method. Significant time effects and substantial fold-increases (immediately post-exercise/pre-exercise) were measured for plasma levels of arachidonic acid (ARA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and 43 of 45 oxylipins. Significant interaction effects (4 trials x 8 time points) were found for plasma ARA (P<0.001) and DHA (P<0.001), but not EPA (P = 0.255), with higher post-exercise values found in the water trial compared to the carbohydrate trials. Significant interaction effects were also measured for 12 of 45 oxylipins. The data supported a strong exercise-induced increase in plasma levels of these oxylipins during the water trial, with carbohydrate ingestion (both bananas types and the sugar beverage) attenuating oxylipin increases, especially those (9 of 12) generated from the cytochrome P-450 (CYP) enzyme system. These trials differences were especially apparent within the first three hours of recovery from the 75-km cycling bout. Prolonged and intensive exercise evoked a transient but robust increase in plasma levels of oxylipins, with a significant attenuation effect linked to acute carbohydrate ingestion for 28% of these, especially those generated through the CYP enzyme system. ClinicalTrials.gov, U.S. National Institutes of Health, NCT02994628.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adult / Analysis / Arachidonic acid / Arachidonic Acid - metabolism / Attenuation / Backup software / Bananas / Beverages / Bicycling / Biological products / Biology and Life Sciences / Biomedical research / Blood Glucose - analysis / Carbohydrates / Chromatography / Cross-Over Studies / Crossovers / Cycles / Cycling / Cyclists / Cytochrome / Cytochrome P-450 / Cytochrome P-450 Enzyme System - metabolism / Cytochrome P450 / Cytochromes P450 / Desaturation / Dietary Carbohydrates - administration & dosage / Docosahexaenoic acid / Docosahexaenoic Acids - metabolism / Eicosapentaenoic acid / Eicosapentaenoic Acid - analogs & derivatives / Eicosapentaenoic Acid - metabolism / Enzymes / Exercise / Fatty acids / Fatty Acids, Omega-3 - metabolism / Female / Health aspects / Humans / Inflammation / Ingestion / Insulin / Laboratories / Linoleic acid / Lipids / Liquid chromatography / Male / Mass spectrometry / Medicine and Health Sciences / Metabolism / Metabolites / Middle Aged / Musa / Nutrition research / Omega 3 fatty acids / Oxidation / Oxidation-reduction reactions / Oxidative stress / Oxygen Consumption / Oxylipins - blood / Physical Sciences / Physiology / Plasma - chemistry / Plasma levels / Polyunsaturated fatty acids / Research design / Scientific imaging / Spectrometry / Sugar / Time Factors / Unsaturated fatty acids / Water / Young Adult