Asset Details
Do you wish to reserve the book?
Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt
Do you wish to request the book?
Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt
2025
Overview
Background
Toxoplasma gondii
is a globally distributed zoonotic parasite that affects both humans and animals, with significant implications for public health and livestock production. The current research aims to update the information on the present prevalence of
T. gondii
and the risk factors associated with the infection in domestic ruminants in Aswan, Egypt, from August 2024 to January 2025, using serological, histopathological, and molecular approaches.
Methods
The blood of 387 domestic ruminants collected during the antemortem examination from four central abattoirs in the Aswan governorate, Upper Egypt, was inspected for the occurrence of anti-
T. gondii
antibodies through a modified agglutination technique. Data were confirmed by a nested polymerase chain reaction that targeted
T. gondii
DNA (
B1
gene). Tissue specimens (heart and diaphragm) from seropositive animals were collected during postmortem examination and subjected to a histopathological and immunohistochemical approach.
Results
The overall occurrence of
T. gondii
was 29.5% (114/387), with seropositivity of 33.5% (52/155), 28.2% (22/78), 23.6% (21/89), and 29.2% (19/65) in cattle, buffalo, sheep, and goats, respectively. The studied risk factors (age, gender, breed, body condition, and location) in this study were detected to be significantly related to the presence of
T. gondii
infection (
p
˂ 0.05). Histopathological examination detected tissue cysts in 38 out of 114 cardiac muscles of seropositive animals and failed to detect any cysts in the diaphragm tissue, indicated by encased, circular to elongated, basophilic cysts with many bradyzoites entrenched in muscle fibers by H&E staining, while showing intense brown granule staining of lymphoblastic cells by immunohistochemistry assay. Nested PCR confirmed the presence of the
B1
gene of
T. gondii
in blood samples of all seropositive animals (100%).
Conclusions
The combined use of serology, PCR, and IHC demonstrates that
T. gondii
is present in slaughtered ruminants in Aswan and that viable tissue cysts are present in edible tissues. These findings highlight a potential risk of zoonotic transmission through the consumption of undercooked meat and emphasize the need for monitoring and control measures to reduce the burden of foodborne toxoplasmosis in Egypt.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Antibodies, Protozoan - blood
/ B1 gene
/ blood
/ Buffalo
/ Cattle
/ Cattle Diseases - epidemiology
/ Cattle Diseases - parasitology
/ Cysts
/ DNA
/ Egypt
/ Ethylenediaminetetraacetic acid
/ Female
/ Food contamination & poisoning
/ gender
/ genes
/ Goat Diseases - epidemiology
/ Goat Diseases - parasitology
/ Goats
/ heart
/ Male
/ Meat
/ Medicine
/ Methods
/ necropsy
/ NPCR
/ Protozoa
/ risk
/ Serology
/ Sheep
/ Sheep Diseases - epidemiology
/ Sheep Diseases - parasitology
/ Toxoplasma - isolation & purification
/ Toxoplasmosis, Animal - epidemiology
/ Toxoplasmosis, Animal - parasitology
/ Toxoplasmosis, Animal - pathology
/ Veterinary Medicine/Veterinary Science
/ Zoology
/ Zoonoses
We currently cannot retrieve any items related to this title. Kindly check back at a later time.